Disclosed are copolymer micelles for simultaneous delivery of Cas9 mRNA and guide RNA for Cas9 CRISPR gene editing. Also disclosed are copolymer micelles for simultaneous delivery of a therapeutic or diagnostic nucleic acid and a cross-linking or alkylating anticancer agent.

Disclosed are copolymer micelles for simultaneous delivery of Cas9 mRNA and guide RNA for Cas9 CRISPR gene editing. Also disclosed are copolymer micelles for simultaneous delivery of a therapeutic or diagnostic nucleic acid and a cross-linking or alkylating anticancer agent.

The object of the present invention is a method for the production of block polymers comprising a firstand a second block comprising the method steps: A) providing a first block having a nucleophilic peptide sequence for afirst transpeptidase enzyme, B) providing a second block having a peptide recognition sequence for the first transpeptidase enzyme, C) linking the first block to the second block by means of the first transpeptidase enzyme, wherein the first and second blocks are independently selected from nanoparticles, non-peptide polymers, and recombinant proteins. Such a production method makes it possible to build block polymers from identical or different blocks in a particularly simple and controlled manner.

The object of the present invention is a method for the production of block polymers comprising a firstand a second block comprising the method steps: A) providing a first block having a nucleophilic peptide sequence for afirst transpeptidase enzyme, B) providing a second block having a peptide recognition sequence for the first transpeptidase enzyme, C) linking the first block to the second block by means of the first transpeptidase enzyme, wherein the first and second blocks are independently selected from nanoparticles, non-peptide polymers, and recombinant proteins. Such a production method makes it possible to build block polymers from identical or different blocks in a particularly simple and controlled manner.

Described herein are methods of lowering the endotoxin content from a polyanionic polymer conjugate. In particular, methods of reducing the endotoxin content from a polyanionic polymer conjugate that can be useful for a variety of drug delivery applications are described herein.

Described herein are methods of lowering the endotoxin content from a polyanionic polymer conjugate. In particular, methods of reducing the endotoxin content from a polyanionic polymer conjugate that can be useful for a variety of drug delivery applications are described herein.

The present invention relates to bone repair material of multivariant amino acid polymer-hydroxyapatite, supportive implants and preparation method. Said restorative material is made of multivariant amino acid polymers consisted with ε-aminocaproic acid and other α-amino acids, together with constituents modified hydroxyapatite, in which the constituent modified hydroxyapatite uses calcium salt as modified constituents that can be accepted in medicine and has a more solubility compared with hydroxyapatite. Modified hydroxyapatite is constructed from said calcium salt and hydroxyapatite, with a mass ratio of (2-20):(98-80), and the content of modified hydroxyapatite is 10-70% based on the mass of said bone repair material; the content of ε-aminocaproic acid in multivariant amino acid polymers is 60-99% based on the total molar quantity of multivariant amino acid polymers.

The invention relates to modified lysines of the formula (I). The invention further relates to polypeptides comprising one or more modified lysine residues, pharmaceutical delivery systems comprising these polypeptides, pharmaceutical compositions containing them, and to their use in therapy.

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The present invention provides peptoid polymers capable of reducing or inhibiting the formation of ice crystals at sub 0° C. temperatures. Also provided are peptoid-peptide hybrids comprising the peptoid polymers provided herein. The peptoid polymers and peptoid-peptide hybrids provided herein are useful for making cryoprotectant solutions. The peptoid polymers, peptoid-peptide hybrids, and cryoprotectant solutions provided herein are useful for making antifreeze solutions, frozen food products, and cosmetic care products. Also provided herein are methods for preserving a tissue, an organ, a cell, or a biological macromolecule using the compositions described herein.

A method of synthesising an amino acid from an unsaturated fatty compound I that includes at least the following steps: cross-metathesis with a short unsaturated compound II, one of compounds I or II comprising a nitrile function and the other of these compounds II or I an ester function, so as to obtain and recover at least one monounsaturated nitrile ester NEU; hydrolysis of the NEU in unsaturated acid nitrile NAU; hydrogenation of the NAU to saturated amino acid AA; and then purification of the AA, if applicable, in particular by crystallisation. Also, a polymer obtained by polymerisation using the amino acid synthesised according to the method.