The present disclosure relates to compounds that are useful as near-infrared fluorescence probes, wherein the compounds include i) a ligand that binds to the active site of carbonic anhydrase, ii) a dye molecule, and iii) a linker molecule that comprises an amino acid, amide, ureido, or polyethylene glycol derivative thereof. The disclosure further describes methods and compositions for making and using the compounds, methods incorporating the compounds, and kits incorporating the compounds.

A near infrared absorbing dye includes a croconium-based compound shown in formula (AI). In formula (AI), ring Z is either an optionally substituted 6-membered ring including no heteroatom or an optionally substituted 5-membered ring including 0 to 3 heteroatoms in the ring, R.sup.1 and R.sup.2, R.sup.2 and R.sup.3, and R.sup.1 and a carbon atom or heteroatom as a part of ring Z may be linked to each other to respectively form a heteroring A, a heteroring B, and a heteroring C together with a nitrogen atom.

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The present disclosure relates to compounds that are useful as near-infrared fluorescence probes, wherein the compounds include i) a ligand that binds to the active site of carbonic anhydrase, ii) a dye molecule, and iii) a linker molecule that comprises an amino acid, amide, ureido, or polyethylene glycol derivative thereof. The disclosure further describes methods and compositions for making and using the compounds, methods incorporating the compounds, and kits incorporating the compounds.

A shrink film comprising a polyethylene-based film having a top surface, a bottom surface, and comprising one or more layers, wherein at least one layer of the polyethylene-based film comprises a low density polyethylene having a density of from 0.917 g/cc to 0.935 g/cc and melt index, I2, of from 0.1 g/10 min to 5 g/10 min, a linear low density polyethylene having a density of from 0.900 g/cc to 0.965 g/cc and melt index, I2, of from 0.05 g/10 min to 15 g/10 min, or combinations thereof, and optionally, a medium density polyethylene, a high density polyethylene, or combinations thereof, and a coating layer disposed on the top surface of the polyethylene-based film, wherein the coating layer comprises an adhesive and a near-infrared absorbent material.

The present invention provides methods, compositions, mixtures and kits utilizing deoxynucleoside triphosphates comprising a 3-O position capped by a group comprising methylenedisulfide as a cleavable protecting group and a detectable label reversibly connected to the nucleobase of said deoxynucleoside. Such compounds provide new possibilities for future sequencing technologies, including but not limited to Sequencing by Synthesis.

The present invention relates to an optical filter containing: an absorption layer containing a near-infrared absorbing dye satisfying the following (i-1) to (i-5), and a transparent resin; and a reflection layer, and satisfying the following (ii-1); (i-1) ?.sub.max(A) TR is in a wavelength range of 760 to 900 nm; (i-2) T.sub.435-480ave (A) TR and T.sub.480-590ave (A) TR are both 90% or more. (i-3) ?.sub.max(A) DCM is in a wavelength range of 760 to 900 nm. (i-4) T.sub.435-480ave (A) DCM is 95% or more and T.sub.480-590ave (A) DCM is 97% or more. (i-5) a difference between T.sub.435-480ave (A) DCM and T.sub.435-480ave (A) TR, and a difference between T.sub.480-590ave (A) DCM and T.sub.480-590ave (A) TR are 10.5% or less. (ii-1) an average transmittance of light in the wavelength range of 800 to 900 nm is 5% or less at an incident angle of 60 degrees.

The present invention provides methods, compositions, mixtures and kits utilizing deoxynucleoside triphosphates comprising a 3-O position capped by a group comprising methylenedisulfide as a cleavable protecting group and a detectable label reversibly connected to the nucleobase of said deoxynucleoside. Such compounds provide new possibilities for future sequencing technologies, including but not limited to Sequencing by Synthesis.

The present invention provides methods, compositions, mixtures and kits utilizing deoxynucleoside triphosphates comprising a 3-O position capped by a group comprising methylenedisulfide as a cleavable protecting group and a detectable label reversibly connected to the nucleobase of said deoxynucleoside. Such compounds provide new possibilities for future sequencing technologies, including but not limited to Sequencing by Synthesis.

The present invention provides methods, compositions, mixtures and kits utilizing deoxynucleoside triphosphates comprising a 3-O position capped by a group comprising methylenedisulfide as a cleavable protecting group and a detectable label reversibly connected to the nucleobase of said deoxynucleoside. Such compounds provide new possibilities for future sequencing technologies, including but not limited to Sequencing by Synthesis.

Fluorescent chromophores nicknamed BOPHY are provided. The chromophores may be readily synthesized in two steps from readily available reagents via the coupling of a pyrrole aldehyde or ketone with hydrazine, followed by reaction with BF.sub.3. The resultant symmetric and dimeric tetracycle is comprised of two BF.sub.2 units in six-member chelate rings, appended by pyrrole units on the periphery. The quantum yield of fluorescence for the unmodified compound and the tetramethyl variant are near unity, with values 95 and 92% respectively in CH.sub.2Cl.sub.2.