The present application relates to the technical field of daily cleaning and washing products, and particularly to a double layer detergent tablet and a preparation method thereof. The double layer detergent tablet includes a color absorbing fiber and a solid detergent adhered to the color absorbing fiber, the solid detergent includes the following components by mass percentage: 8-30% of polyvinyl alcohol, 10-40% of surfactant, 0.5-5% of polyvinylpyrrolidone, 1-5% of glycerin, 0.5-5% of water softener, 0.1-1% of plant essence, 14-79.9% of deionized water. The surfactant is a mixture of an anionic surfactant and a non-ionic surfactant; the anionic surfactant is a mixture of sodium coco-sulfate and sodium alpha-olefin sulfonate; and the non-ionic surfactant is a mixture of alkyl glycoside, fatty alcohol polyoxyethylene ether, isomeric alcohol polyoxyethylene ether and fatty acid methyl ester ethoxy compound.

Bioactive coatings that include a base and a protein associated with the base for actively promoting the removal of organic stains are provided. In aspects, bioactive coatings that are stabilized against inactivation by weathering are provided including a base associated with a chemically modified enzyme, and, optionally a first polyoxyethylene present in the base and independent of the enzyme. The coatings are optionally overlayered onto a substrate to form an active coating facilitating the removal of organic stains or organic material from food, insects, or the environment.

Bioactive coatings that include a base and a protein associated with the base for actively promoting the removal of organic stains are provided. In aspects, bioactive coatings that are stabilized against inactivation by weathering are provided including a base associated with a chemically modified enzyme, and, optionally a first polyoxyethylene present in the base and independent of the enzyme. The coatings are optionally overlayered onto a substrate to form an active coating facilitating the removal of organic stains or organic material from food, insects, or the environment.

Provided herein is a method of at least partly removing a biological deposit, such as a dag, from the skin of an animal. The method includes administering to the biological deposit an effective amount of a composition containing a keratinase, and optionally one or both of a reducing agent and a surfactant. Also provided is a composition for use in the aforementioned method as well as a method of making same.

The present invention relates to isolated polypeptides having proteolytic activity and polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors and host cells comprising the polynucleotides, compositions comprising the polypeptides, and methods of producing and using the polypeptides.

A fluid washing composition with four separate components and laundry washing kit thereof is disclosed. The four components are housed in respective independent tanks of a laundry washing machine equipped with a program for selecting, dosing and differentiated delivering. Component 1 is a detergent formulation with a pH of 4.0-12.0, with high detergent effect in washing programs at temperatures of 40°-60° C., having a specific action on cotton fibers, particularly, whites. Component 2 is a detergent formulation with a pH of 8.0-12.0, with high detergent effect in washing programs at temperatures of 15°-40° C., having a specific action on synthetic, colored and animal-origin fibers. Component 3 is an enzyme-based stain-removing formulation including protease and lipase and possibly amylase with a pH of 5.0-6.0, stabilized with sorbitol and calcium chloride. Component 4 is an after-wash conditioning formulation, with a pH of 2.0-4.0.

A fluid washing composition with four separate components and laundry washing kit thereof is disclosed. The four components are housed in respective independent tanks of a laundry washing machine equipped with a program for selecting, dosing and differentiated delivering. Component 1 is a detergent formulation with a pH of 4.0-12.0, with high detergent effect in washing programs at temperatures of 40°-60° C., having a specific action on cotton fibers, particularly, whites. Component 2 is a detergent formulation with a pH of 8.0-12.0, with high detergent effect in washing programs at temperatures of 15°-40° C., having a specific action on synthetic, colored and animal-origin fibers. Component 3 is an enzyme-based stain-removing formulation including protease and lipase and possibly amylase with a pH of 5.0-6.0, stabilized with sorbitol and calcium chloride. Component 4 is an after-wash conditioning formulation, with a pH of 2.0-4.0.

The invention provides a liquid boron-free enzyme composition, comprising aliphatic 1,2-diols, which exhibit excellent physical and microbial stability.

Cleaning or treatment compositions including DNase variants and cleaning adjunct. Methods of treating surfaces such as fabrics by contacting the surface with an aqueous wash liquor having the cleaning composition therein. The compositions and methods are particularly for improving whiteness of a fabric, improved soil removal from a fabric, for malodour removal from a fabric, for anti-wrinkle benefits, anti-redeposition benefits and/or for improved drying of a fabric.

The present disclosure relates to proteases having an amino acid sequence with at least 70% sequence identity to the amino acid sequence given in SEQ ID No. 1, across its whole length, and comprising an amino acid substitution on at least one of the positions P9, Q10, Q62, L82, P86, N130, T141, N187, S236 or T253, relating in each case to the numbering according to SEQ ID No. 1. The present disclosure also relates to the production and use thereof. Said type of proteases have a very good cleaning performance.