The subject invention provides compositions and methods for reducing the carbon footprint of producing livestock. Microbe-based soil treatment compositions reduce greenhouse gas emissions from producing livestock feed, and in turn, improve the health and productivity of livestock animals.

The subject invention provides materials and methods for reducing deleterious atmospheric gases, such as greenhouse gases (GHGs) by enhancing utilization and storage of carbon in plants, as well as increasing the sequestration of carbon in plant and soil matter in the form of degradation-resistant organic polymers.

A novel isolated Pichia stipitis strain is provided. The strain is capable of fermenting at least a pentose sugar in the presence of one or more inhibitory substances to produce ethanol. A method of utilizing the strain to produce ethanol is also provided.

Strains of yeast genetically engineered to produce increased amounts of non-hydroxylated collagen or hydroxylated collagen are described. An all-in-one vector including the DNA necessary to produce collagen, promotors, and hydroxylating enzymes is also described. Methods for producing non-hydroxylated or hydroxylated collagen are also provided.

The invention relates to preparation of fermented food products, including dairy or dairy analogue products, with Pichia kluyveri and lactic acid bacteria in the presence of carbohydrate(s) which is sucrose, fructose, glucose or mixtures thereof. Fermentation of such products can be made using a starter culture composition comprising Pichia kluyveri strain(s) and lactic acid bacteria strain(s). The composition optionally comprises carbohydrate(s) which is sucrose, fructose and/or glucose.

An oleaginous yeast strain of the species Trichosporon oleaginosus, deposited on May 28, 2020 at Leibniz-Institut DSMZ, deposit number DSM 33530 can be used in the production of lipids by fermentation and was obtained by mutagenesis and subsequent selection from the oleaginous yeast strain of the species Trichosporon oleaginosus DSM 32508. The strain DSM 33530 is characterized by high thermotolerance, such as to allow the fermentation temperature to be increased compared to conventional processes and still guarantee the vital functions of the microorganism during fermentation itself.

The present invention resides in the discovery that altered abundance of fungal species in the gut and feces of both fecal microbiota transplantation (FMT) donors and recipients may influence the outcome of FMT treatment. Thus, novel methods are provided for identifying subjects as suitable donor or recipients for FMT, for assessing the likelihood of FMT treatment success, and for enhancing FMT treatment efficacy. Also provided are kits and compositions for FMT with enhanced efficacy.

[Problem] To provide a new technology that enables reducibility to be maintained for a prolonged period of time.

[Solution] A composition having reducing ability, comprising a substance having reducing ability and a wet ball mill-treated product of yeast cell wall and/or a hydrothermal reaction-treated product of yeast cell wall.

The invention relates to method for the efficient expression and purification and application of a recombinant fusion protein of MANNase and homologues thereof and GLP-1. Through genetic recombination technology, soluble proteins are obtained by using high density fermentation of Pichia pastoris to induce secretory expression, followed by isolation and purification by filtration and concentration to obtain high yield of target proteins. The high fermentation expression and simple isolation steps solve the limitations of the current GLP-1 analogs such as low drug yield, high cost and the need for frequent injections. More importantly, the fusion protein has hypoglycemic effect not only by injection, but also by oral administration for hypoglycemia and weight reduction, which has good application value in obese and pre-diabetic patients. It also provides a basis for further research on the mechanism of hypoglycemia and weight loss of mannanase and homologues thereof with GLP-1 recombinant fusion protein.

A novel isolated Pichia stipitis strain is provided. The strain is capable of fermenting at least a pentose sugar in the presence of one or more inhibitory substances to produce ethanol. A method of utilizing the strain to produce ethanol is also provided.