Patent classifications
C12N1/30
Polyhydroxyalkanoate production methods and systems for same
Several embodiments of the invention relate generally to a system and methods for the treatment of gaseous emissions comprising methane and one or more non-methane compounds that can influence the metabolism of methane-oxidizing microorganisms. In several embodiments, there is provided a system and methods for the treatment of methane emissions through the use of methanotrophic microorganisms to generate functionally consistent and harvestable products. Certain embodiments of the invention are particularly advantageous because they reduce environmentally-destructive methane emissions and produce harvestable end-products.
GAS-FED FERMENTATION REACTORS, SYSTEMS AND PROCESSES
Reactors, systems and processes for the production of biomass by culturing microorganisms in aqueous liquid culture medium circulating inner loop reactor which utilize nonvertical pressure reduction zones are described. Recovery and processing of the culture microorganisms to obtain products, such as proteins or hydrocarbons is described.
GAS-FED FERMENTATION REACTORS, SYSTEMS AND PROCESSES
Reactors, systems and processes for the production of biomass by culturing microorganisms in aqueous liquid culture medium circulating inner loop reactor which utilize nonvertical pressure reduction zones are described. Recovery and processing of the culture microorganisms to obtain products, such as proteins or hydrocarbons is described.
METHOD FOR CONTROLLING A FERMENTATION PROCESS
The present invention relates to a fermentation process for the fermentation of at least one microorganism, wherein the fermentation process comprises the steps of (a) allowing a fermentation broth comprising the at least one microorganism to flow in the fermentation reactor; (b) supplying a carbon-substrate to the fermentation reactor allowing the gaseous carbon-substrate to be dissolved, or partly dissolved, in the fermentation broth; (c) supplying a nitrogen-substrate to the fermentation reactor allowing the gaseous nitrogen-substrate to be dissolved, or partly dissolved, in the fermentation broth; and (d) maintaining a nitrate concentration of the fermentation broth below 0.035 g/l, and/or maintaining a nitrate concentration of the fermentation broth below 0.01 g nitrate/g biomass; wherein the at least one methanotrophic organism comprises at least one methanotrophic microorganism.
METHOD FOR CONTROLLING A FERMENTATION PROCESS
The present invention relates to a fermentation process for the fermentation of at least one microorganism, wherein the fermentation process comprises the steps of (a) allowing a fermentation broth comprising the at least one microorganism to flow in the fermentation reactor; (b) supplying a carbon-substrate to the fermentation reactor allowing the gaseous carbon-substrate to be dissolved, or partly dissolved, in the fermentation broth; (c) supplying a nitrogen-substrate to the fermentation reactor allowing the gaseous nitrogen-substrate to be dissolved, or partly dissolved, in the fermentation broth; and (d) maintaining a nitrate concentration of the fermentation broth below 0.035 g/l, and/or maintaining a nitrate concentration of the fermentation broth below 0.01 g nitrate/g biomass; wherein the at least one methanotrophic organism comprises at least one methanotrophic microorganism.
METHOD FOR OPTIMIZING A FERMENTATION PROCESS
The present invention relates to a process for cultivating one or more microorganisms capable of metabolising methane, the process comprises the steps of: (i) adding a fermentation medium to a fermentation reactor; (ii) adding the one or more microorganism to a fermentation reactor, providing an inoculated fermentation medium, wherein the one or more microorganism does not include a recombinant microorganism; (iii) adding a C1-C5 carbon source, e.g. methane, to the fermentation reactor and/or the inoculated fermentation medium during the fermentation of the one or more microorganisms; and (iv) optionally, adding oxygen to the fermentation reactor and/or the inoculated fermentation medium during the fermentation of the one or more microorganisms, wherein the oxygen is added to the fermentation reactor and/or the inoculated fermentation medium to provide a content of undissolved oxygen in the fermentation reactor and/or a content of gaseous oxygen in an exhaust gas is at most 10% (vol/vol).
METHOD FOR OPTIMIZING A FERMENTATION PROCESS
The present invention relates to a process for cultivating one or more microorganisms capable of metabolising methane, the process comprises the steps of: (i) adding a fermentation medium to a fermentation reactor; (ii) adding the one or more microorganism to a fermentation reactor, providing an inoculated fermentation medium, wherein the one or more microorganism does not include a recombinant microorganism; (iii) adding a C1-C5 carbon source, e.g. methane, to the fermentation reactor and/or the inoculated fermentation medium during the fermentation of the one or more microorganisms; and (iv) optionally, adding oxygen to the fermentation reactor and/or the inoculated fermentation medium during the fermentation of the one or more microorganisms, wherein the oxygen is added to the fermentation reactor and/or the inoculated fermentation medium to provide a content of undissolved oxygen in the fermentation reactor and/or a content of gaseous oxygen in an exhaust gas is at most 10% (vol/vol).
SYSTEM AND METHOD FOR METHANE BIODEGRADATION
Biodegradation media placed in, around, and/or above a methane source reduces the quantum of methane and other alkane gases such as ethane, propane, and butane released into the atmosphere under diverse and fluctuating environmental conditions over a sustainable and/or extended duration. Non-biodegradable material configured for methane biodegradation possesses enhanced drainage of precipitation, improved gas transmission and gas exchange, moisture retention, and a nutrient sustainability.
SYSTEM AND METHOD FOR METHANE BIODEGRADATION
Biodegradation media placed in, around, and/or above a methane source reduces the quantum of methane and other alkane gases such as ethane, propane, and butane released into the atmosphere under diverse and fluctuating environmental conditions over a sustainable and/or extended duration. Non-biodegradable material configured for methane biodegradation possesses enhanced drainage of precipitation, improved gas transmission and gas exchange, moisture retention, and a nutrient sustainability.
POLYHYDROXYALKANOATE PRODUCTION METHODS AND SYSTEMS FOR SAME
Several embodiments of the invention relate generally to a system and methods for the treatment of gaseous emissions comprising methane and one or more non-methane compounds that can influence the metabolism of methane-oxidizing microorganisms. In several embodiments, there is provided a system and methods for the treatment of methane emissions through the use of methanotrophic microorganisms to generate functionally consistent and harvestable products. Certain embodiments of the invention are particularly advantageous because they reduce environmentally-destructive methane emissions and produce harvestable end-products.