Non-human animal cells and non-human animals comprising a humanized Asgr1 locus and methods of using such non-human animal cells and non-human animals are provided. Non-human animal cells or non-human animals comprising a humanized Asgr1 locus express a human ASGR1 protein or an Asgr1 protein, fragments of which are from human ASGR1. Methods are provided for using such non-human animals comprising a humanized Asgr1 locus to assess in vivo efficacy of human-ASGR1-mediated delivery of therapeutic molecules or therapeutic complexes to the liver and to assess the efficacy of therapeutic molecules or therapeutic complexes acting via human-ASGR1-mediated mechanisms.

The present invention relates to the field of pluripotent stem cell culture and methods facilitate pluripotent stem cell culture at industrial levels.

The invention encompasses methods of increasing the rate of genetic progress or generating inbred lines in a non-human mammalian species comprising the use of gametes derived from embryos of the non-human mammalian species.

Described herein are methods and related compositions for inducing differentiation of human pluripotent stem cells (hPSCs) into hemogenic endothelium with pan-myeloid potential or restricted potential, by forced expression in the hPSCs of a combination of transcription factors as described herein.

An embryo, gamete or stem cell culture medium comprising: a) acetyl-carnitine at a concentration of about 5 to about 50 M; and b) lipoic acid or a derivative thereof at a concentration of about 2.5 to about 40 M. The culture medium may optionally further comprises acetyl-cysteine at a concentration of about 5 to about 50 M.

A determination method of non-destructively and easily determining a state of an aggregate of a plurality of cells formed by three-dimensional culture is provided. A determination method according to the disclosed technology includes generating a phase difference image of an aggregate of a plurality of cells from a hologram obtained by imaging the aggregate, deriving a phase difference amount density by dividing a total phase difference amount that is a value obtained by integrating a phase difference amount of each of a plurality of pixels constituting the phase difference image by a volume of the aggregate, and determining a state of the aggregate on the basis of a time transition of the phase difference amount density.

The subject matter of this specification can be embodied in, among other things, a method for controlling an incubator that includes receiving by a controller a temperature profile, and varying by the controller a temperature of an incubator based on the temperature profile.

Described herein are methods and related compositions for inducing differentiation of human pluripotent stem cells (hPSCs) into hemogenic endothelium with pan-myeloid potential or restricted potential, by forced expression in the hPSCs of a combination of transcription factors as described herein.

Methods are described for differentiating stem and post-natal cells into sex hormone-producing cells that can be administered to a patient autologously or allogeneically in order to maintain in balance, or rebalance, their hypothalamic-pituitary-gonadal (HPG) axis.

The present invention relates to the handling of biological samples, for example, the holding, manipulating and culturing of biological samples. In one form the invention provides an overlay encapsulant for an in vitro cell culture comprising a synthetic compound and in another aspect the invention provides methods of temporarily encapsulating an in vitro cell culture comprising a synthetic compound. The invention has use in relation to the culturing and more particularly the encapsulation of biological samples, such as for example zygotes, embryos, oocytes, stem cells, sperm located in a culturing space, relevant pluripotent derivative(s) and/or differentiated progeny, intact or dispersed tissue and/or intact organism(s).