The present invention includes a method of preparing differentiated endodermal cells comprising growing human pluripotent stem cells (hPSC) under serum free conditions for 3 days on a layer of fibronectin or a gelatinous protein layer in the presence of high activin/TGF- with a GSK3 inhibitor and a PI3K/mTORC inhibitor in a basal differentiation medium; adding a knock out serum replacement to the media starting on day 4 through day 7; and harvesting the endodermal cells grown therein.

The present invention provides a method of obtaining aggregates containing a rostral hypothalamus tissue and a rostral head ectodermal tissue, a hypophysis precursor tissue and a hypophysis hormone producing cell, by using a serum-free medium (preferably substantially free of growth factor and insulins), forming homogeneous aggregates of stem cells from pluripotent stem cells such as ES cell and the like, which are plated at a high cell concentration, and subjecting the formed aggregates to floating-culture.

The present invention relates to differentiation of stem cells into a homogeneous endocrine progenitor cell population suitable for further differentiation into pancreatic beta-cells.

The present invention provides methods for obtaining NGN3/NKX2.2 double positive endocrine progenitor cells by exposing precursor cells to a TGF- type I receptor inhibitor, a BMP antagonist, an adenylate cyclase activator and nicotinamide and/or exposing to the precursor cells to a selection of small molecules.

The present invention provides, among other things, compositions including a plurality of enterocytes, a plurality of fibroblasts, a plurality of Goblet cells, a plurality of Paneth cells, a plurality of enteroendocrine cells, and a silk fibroin scaffold, wherein the composition exhibits one or more of tight junction formation, microvilli polarization, digestive enzyme secretion, and low oxygen tension and methods of making and using the same. In some embodiments, the composition exhibits one or more of tight junction maintenance, maintenance of microvilli polarization, digestive enzyme secretion, and low oxygen tension for at least 10 days.

The present invention provides devices and methods for delivering a population of cells or a therapeutic agent to a subject in need thereof.

The present disclosure provides a medium for cells, such as human stem cells including human induced pluripotent stem cells (iPScs) which medium enhances the formation of definitive endoderm (DE) which in turn can be subsequently directed and differentiated into mature cell types, e.g., pancreas insulin producing cells.

Disclosed herein are methods for directing the differentiation of stem cells towards a specific cell type.

The present invention provides devices and methods for delivering a population of cells or a therapeutic agent to a subject in need thereof.

The invention relates to methods of inducing insulin production in delta-cells and/or converting delta-cells into insulin producing cells, as well as methods of preventing and/or treating diabetes and agents and compositions useful in said methods.

The present invention provides devices and methods for delivering a population of cells or a therapeutic agent to a subject in need thereof.