Disclosed are methods of treating aging effects on a subject skin, comprising contacting the skin with a skin microbiome complex, including topical compositions containing YM Microbiome Complex and Advanced Probiotic Complex, methods of treatment using the compositions and methods of preparing the compositions for preventing, slowing, and reversing skin aging. Also disclosed are related formulations and methods of skin microbiome transplantation.

The present invention provides: a composition for reconstituting human skin tissue having hair follicles, the composition characterized by containing human epidermal cells and human dermal cells, the human dermal cells containing cell groups of human hair papilla derived from non-embryos via spheroid formation; and a human skin tissue model animal to which said composition is applied. The invention also provides a method for producing said composition and animal.

The present invention relates to a method of promoting skin rejuvenation in a subject. The method comprises administering to the subject an effective amount of a cellular extract of epithelial or mesenchymal stem/progenitor cells isolated from the amniotic membrane of the umbilical cord, wherein the cellular extract contains growth factors and peptides and is in the form of a supernatant into which the epithelial or mesenchymal stem/progenitor cells secrete the growth factors and peptides.

A microfluidic device is contemplated comprising an open-top cavity with structural anchors on the vertical wall surfaces that serve to prevent gel shrinkage-induced delamination, a porous membrane (optionally stretchable) positioned in the middle over a microfluidic channel(s). The device is particularly suited to the growth of cells mimicking dermal layers.

The present disclosure relates to methods for culturing human epidermal keratinocytes. When keratinocytes are cultured on plates coated with a laminin containing an alpha-4 chain or an alpha-5 chain, in a xeno-free, chemically defined cell culture medium, they expand efficiently in vitro. Useful cell culture kits for culturing keratinocytes are also described herein, as are methods of using such cells for treatment of burns or chronic wounds.

The present invention relates to artificial skin having structure and properties by age, and more specifically to artificial skin having structure and properties, for which effective analysis and application are possible on the basis of age of the skin, as the human skin can be replicated according to age by simple control in the internal structure of properties such as height and width, and a method for manufacturing the same.

Disclosed herein are synthetic leathers, artificial epidermal layers, artificial dermal layers, layered structures, products produced therefrom and methods of producing the same.

Provided are live, artificial, skin substitute products and methods of making and using the same, such as for wound treatment and compound testing, including compound testing for efficacy, toxicity, penetration, irritation and/or metabolism testing of drug candidates or compositions such as cosmetics.

An object of the present invention is to provide a new means for suppressing inhibition of proliferation of keratinocytes even when the keratinocytes start to come into contact with each other. The present invention solves the above problem by culturing keratinocytes in contact with a common medium with fibroblasts treated with a composition containing a specific compound.

Disclosed herein are synthetic leathers, artificial epidermal layers, artificial dermal layers, layered structures, products produced therefrom and methods of producing the same.