A method for producing soluble PDIA2, compositions containing it, and methods for its use.

This disclosure provides various TcBuster transposases and transposons, systems, and methods of use.

The present disclosure concerns agents and their use in the treatment of endocrine, nutritional and/or metabolic diseases in a mammal. The disclosure furthermore concerns novel peptides.

The invention relates to the field of cell culture technology. It concerns the knockdown, using RNA interference, or gene knockout, of activating transcription factor 6 beta (ATF6B), or the combination of ceramide synthase 2 (CERS2) and TBC1 domain family member 20 (TBC1 D20) proteins, which play central roles in the cellular secretion pathway. This downregulation leads to improved secretion of biopharmaceutically relevant products produced in mammalian cells. The invention specifically relates to mammalian cells having enhanced secretion of a recombinant therapeutic protein compared to a control cell, a method of producing said mammalian cell, a method for the production of a recombinant secreted therapeutic protein and the use of said mammalian cell for increasing the yield of a recombinant secreted therapeutic protein.

The present disclosure concerns agents and their use in the treatment of endocrine, nutritional and/or metabolic diseases in a mammal. The disclosure furthermore concerns novel peptides.

The present invention is directed to fluorescent molecular sensor based on Thiazole Orange for protein detection. Interaction of the protein target with the molecular sensors of this invention results in a significant increase in the fluorescence emission. The generation of light output signal enables one to detect protein biomarkers associated with different diseases or detecting the protein of interest also in living cells.

A metabolic enzyme-induced micro-nano plastic particle biodegradation method and a product analysis method therefor are disclosed. The biodegradation method includes the following steps: step (1), cutting an appropriate quantity of plastic products into small pieces; step (2), placing the plastic products in a ball mill tank and milling the plastic products in vacuum in a ball mill; step (3), weighing and dispersing the milled plastic sample with water to prepare a dispersion with a concentration; step (4), mixing the micro-nano plastic dispersion with a metabolic enzyme solution, and placing the mixed solution in a vortex oscillator for mixing well; and step (5), incubating the mixed solution in a waterproof incubator. The present invention provides for the first time a method for biodegradation and metabolism of micro-nano plastics under mild conditions and under the induction of a metabolic enzyme, namely, glutathione S-transferase.

Fusion proteins containing active agonist or antagonist fragments of parathyroid hormone (PTH) and parathyroid hormone related peptide (PTHrP) coupled to a collagen-binding domain are presented. The fusion proteins can be used to promote bone growth, to promote hair growth, to prevent cancer metastasis to bone, to promote immune reconstitution with a bone marrow stem cell transplant, to promote mobilization of bone marrow stem cells for collection for autologous stem cell transplant, and to treat renal osteodystrophy. Pharmaceutical agents comprising a collagen-binding polypeptide segment linked to a non-peptidyl PTH/PTHrP receptor agonist or antagonist are also presented.

A metabolic enzyme-induced micro-nano plastic particle biodegradation method and a product analysis method therefor are disclosed. The biodegradation method includes the following steps: step (1), cutting an appropriate quantity of plastic products into small pieces; step (2), placing the plastic products in a ball mill tank and milling the plastic products in vacuum in a ball mill; step (3), weighing and dispersing the milled plastic sample with water to prepare a dispersion with a concentration; step (4), mixing the micro-nano plastic dispersion with a metabolic enzyme solution, and placing the mixed solution in a vortex oscillator for mixing well; and step (5), incubating the mixed solution in a waterproof incubator. The present invention provides for the first time a method for biodegradation and metabolism of micro-nano plastics under mild conditions and under the induction of a metabolic enzyme, namely, glutathione S-transferase.

The invention provides proteins from group B streptococcus (Streptococcus agalactiae) and group A streptococcus (Streptococcus pyogenes), including amino acid sequences and the corresponding nucleotide sequences. Data are given to show that the proteins are useful antigens for vaccines, immunogenic compositions, and/or diagnostics. The proteins are also targets for antibiotics.