Provided are a taxadiene synthase TcTS2, an encoding nucleotide sequence and use thereof. The amino acid sequence of TcTS2 includes or consists of: (a) an amino acid sequence represented by SEQ ID NO: 1; or (b) a functional homologous sequence having at least 80% sequence similarity with the amino acid sequence represented by SEQ ID NO: 1; or (c) an amino acid sequence having TcTS2 activity with addition, deletion, or substitution of one or more amino acids in the amino acid sequence represented by SEQ ID NO: 1. TcTS2 and the nucleotide sequence encoding the TcTS2 provide new gene resources for improving the yield of taxol, and they may be used for modifying chassis hosts by plant genetic engineering and metabolic engineering strategies to produce taxol and the intermediates thereof etc., thereby having significant economic and social value.

Provided are a taxadiene synthase TcTS2, an encoding nucleotide sequence and use thereof. The amino acid sequence of TcTS2 includes or consists of: (a) an amino acid sequence represented by SEQ ID NO: 1; or (b) a functional homologous sequence having at least 80% sequence similarity with the amino acid sequence represented by SEQ ID NO: 1; or (c) an amino acid sequence having TcTS2 activity with addition, deletion, or substitution of one or more amino acids in the amino acid sequence represented by SEQ ID NO: 1. TcTS2 and the nucleotide sequence encoding the TcTS2 provide new gene resources for improving the yield of taxol, and they may be used for modifying chassis hosts by plant genetic engineering and metabolic engineering strategies to produce taxol and the intermediates thereof etc., thereby having significant economic and social value.

The present invention relates to a strain which has increased glutamine productivity due to being transformed with a vector containing a nucleotide sequence that encodes a glutamine synthetase consisting of the amino acid sequence of SEQ ID NO: 1.

The present invention relates to a strain which has increased glutamine productivity due to being transformed with a vector containing a nucleotide sequence that encodes a glutamine synthetase consisting of the amino acid sequence of SEQ ID NO: 1.

The present disclosure provides recombinant nucleic acids comprising one or more polynucleotides encoding a polypeptide; viruses comprising the recombinant nucleic acids; compositions comprising the recombinant nucleic acids and/or viruses; methods of their use; and articles of manufacture or kits thereof.

The present disclosure provides recombinant nucleic acids comprising one or more polynucleotides encoding a polypeptide; viruses comprising the recombinant nucleic acids; compositions comprising the recombinant nucleic acids and/or viruses; methods of their use; and articles of manufacture or kits thereof.

The invention provides genetically engineered microorganisms and methods for the biological production of ethylene glycol and precursors of ethylene glycol. In particular, the microorganism of the invention produces ethylene glycol or a precursor of ethylene glycol through one or more of 5,10-methylenetetrahydrofolate, oxaloacetate, citrate, malate, and glycine. The invention further provides compositions comprising ethylene glycol or polymers of ethylene glycol such as polyethylene terephthalate.

The invention provides genetically engineered microorganisms and methods for the biological production of ethylene glycol and precursors of ethylene glycol. In particular, the microorganism of the invention produces ethylene glycol or a precursor of ethylene glycol through one or more of 5,10-methylenetetrahydrofolate, oxaloacetate, citrate, malate, and glycine. The invention further provides compositions comprising ethylene glycol or polymers of ethylene glycol such as polyethylene terephthalate.

Embodiments provide a modified microbe capable of growing in or fermenting a solution, or lignocellulosic hydrolysate, comprising ferulic acid and/or coniferyl aldehyde. The microbe has one or more modifications to provide: (a) a decrease in copy number or expression of a BNA7 gene; (b) an increase in copy number or expression of one or more pentose phosphate pathway genes; and/or (c) localization of one or more products of the pentose phosphate pathway genes to the mitochondria or endoplasmic reticulum. Also provided is a microbe having modified expression or copy number of BNA7 and/or one or more of the pentose phosphate pathway genes. The pentose phosphate pathway genes may in certain embodiments be selected from at least one of ZWF1, TKL1, RPE1 and GND1. Also provided is a method for fermenting a substrate comprising ferulic acid and/or coniferyl aldehyde to produce a fermentation product.

The present disclosure identifies pathways, mechanisms, systems and methods to confer chemoautotrophic production of carbon-based products of interest, such as sugars, alcohols, chemicals, amino acids, polymers, fatty acids and their derivatives, hydrocarbons, isoprenoids, and intermediates thereof, in organisms such that these organisms efficiently convert inorganic carbon to organic carbon-based products of interest using inorganic energy, such as formate, and in particular the use of organisms for the commercial production of various carbon-based products of interest.