The current invention reports the use of meta-tyrosine for increasing the specific productivity of a eukaryotic cell that produces/expresses a polypeptide. In the current method it is not necessary to perform a temperature-, osmolality- or pH shift or to add drugs like valproic acid or sodium butyrate to modulate the specific productivity of the cultivated cells. The method does not affect cell viability or product titer.

The present invention relates to a method for manipulating the high mannose glycoform content of recombinant glycoproteins by regulating ornithine metabolism during cell culture.

A system and method for generating an incubated bacteria solution by heating a nutrient germinant composition and bacteria, including at least one species in spore form, to a preferred temperature a range of 35-50 C. for 2-60 minutes using exothermic chemical reaction heat. An incubated bacteria solution is preferably generated at or near a point-of-use in an aquaculture, agriculture, wastewater, or environmental remediation application. The nutrient-germinant composition comprises L-amino acids, optionally D-glucose and/or D-fructose, a buffer, an industrial preservative, and may include bacteria spores (preferably of one or more Bacillus species) or they may be separately combined for incubation. A first chemical contained in a pouch is activated by contact with a second chemical, water, or air in a flameless heater to initiate exothermic reaction to provide incubation heat. A potable, single-use incubation bag is configured to hold the flameless heater and a container of nutrient germinant composition and spores.

The present invention relates in part to methods for producing tissue-specific cells from patient samples, and to tissue-specific cells produced using these methods. Methods for reprogramming cells using RNA are disclosed. Therapeutics comprising cells produced using these methods are also disclosed.

The present invention relates to a method for manipulating the high mannose glycoform content of recombinant glycoproteins by regulating ornithine metabolism during cell culture.

The present disclosure relates to tissue engineering, and more particularly to a method for treating or repairing rotator cuff or other tendon tears or damage using scaffold-free, 3-dimensional engineered tendon constructs.

The present invention provides a method for producing a retinal progenitor cell, including (1) a first step of subjecting pluripotent stem cells to floating culture in a serum-free medium to form an aggregate of pluripotent stem cells, and (2) a second step of subjecting the aggregate formed in step (1) to floating culture in a serum-free medium or serum-containing medium each being free of a substance acting on the Sonic hedgehog signal transduction pathway but containing a substance acting on the BMP signal transduction pathway, thereby obtaining an aggregate containing retinal progenitor cells.

In some aspects, described herein are cell culture media that are useful for in vitro culture of mammalian cells. The culture media contain a variety of small organic compounds that are found in normal adult human blood. Also described are methods of using the culture media for a variety of purposes. Also described are methods of treating cancer.

Compositions and methods are described herein for transdifferentiation of multipotent stromal cells into cells that can express insulin.

Compositions and methods are described herein for transdifferentiation of multipotent stromal cells into cells that can express insulin.