A cell for treating neurodegenerative disease treated with angelica extract is provided. The pharmaceutical composition comprises the cell for treating neurodegenerative disease and can significantly increase and recover the number of dopaminergic neurons to achieve the goal for treating neurodegenerative disease.

Provided is a new type serum-free medium. The medium comprises: DMEM with high glucose (the content of glucose being 4.5 g/L), B27, recombinant human basic fibrolast growth factor (b-FGF), nicotinamide, N-2, vinblastine III (conophylline), non-essential amino acid (NEAA), heparin, epidermal growth factor (EGF), hepatocyte growth factor (HGF), a serum replacement (SR), an insulin-transferrin-selenium complex (ITS), and pentagastrin. Inducing differentiation of mesenchymal stem cells into insulin-secretion-like cells can be achieved in six days in one step using the medium.

The present disclosure relates to a composition for inducing differentiation into beige adipocytes, which contains an exosome derived from stem cells differentiating into beige adipocytes as an active ingredient, a pharmaceutical composition, a health functional food and a differentiation medium composition containing the composition and a method for inducing differentiation into beige adipocytes using the same.

A method of generating a population of cells useful for treating a nerve disease or disorder in a subject, the method comprising up-regulating a level of at least one exogenous miRNA in mesenchymal stem cells (MSCs) and/or down-regulating a level of at least one miRNA using a polynucleotide agent that hybridizes to the miRNA, thereby generating the population of cells useful for treating the nerve disease or disorder. Isolated populations of cells with an astrocytic phenotype generated thereby and uses thereof are also provided.

A serum-free culture method for human umbilical cord mesenchymal stem cells (hUC-MSC), said method using a step-by-step method to culture hUC-MSC: first using a TME culture medium for culturing for 3-4 hours to promote hUC-MSC adherence, and then switching to a TMD culture medium for rapid amplification.

Methods, devices and systems are disclosed for vibration induced tissue engineering. Apparatuses, devices, systems and methods are provided for engineering a tissue from stem cells using vibratory signals. A tissue engineering apparatus, device, or system can include a transmitter configured to transmit a signal to a receiver, a receiver configured to receive a signal from a transmitter, a holding structure configured to hold one or more mesenchymal stem cells (MSC), and a vibratory actuator in communication with the receiver and configured to generate a vibratory signal that is applied to the MSC.

The invention relates to a method of producing mesenchymal stem cell derived exosomes comprising the steps of,

performing a method of producing a clonal mesenchymal stem cell line capable of producing exosomes (MSC-derived extracellular vesicles) comprising the steps of,

providing human induced pluripotent stem cells (hiPSCs),

generating therefrom an immortalized clonal cell line of mesenchymal stem cells (IMSCs),

characterizing the potential of the IMSCs to produce exosomes,

cultivating the desired IMSCs such that the IMSCs produce exosomes,

isolating the exosomes.

The invention relates to extracellular vesicles or exosomes, produced according to the invention.

The invention relates to the use of these EVs as a medicament and in a pharmaceutical composition.

The invention relates to mesenchymal stem cell derived exosomes produced by a method comprising the steps of, performing a method of producing a clonal mesenchymal stem cell line capable of producing exosomes (MSC-derived extracellular vesicles) comprising the steps of, providing human induced pluripotent stem cells (hiPSCs), generating therefrom an immortalized clonal cell line of mesenchymal stem cells (IMSCs), characterizing the potential of the IMSCs to produce exosomes, cultivating the desired IMSCs such that the IMSCs produce exosomes, isolating the exosomes.

The present invention relates to a medium composition containing an Ecklonia cava extract for dedifferentiation an induced pluripotent stem cell. Also, the present invention relates to a method for differentiating an induced pluripotent stem cell produced by using the medium composition into hepatocytes. When using the medium composition according to the present invention, induced pluripotent stem cells using mesenchymal stem cells can be produced efficiently, and the pluripotent stem cells which have been produced can be useful as a cell treatment agent by being capable of being differentiated into hepatocytes.

The present invention relates to the generation of a chondrocyte using mesenchymal stem/progenitor cells obtained from the amniotic membrane of umbilical cord and therapeutic uses of such chondrocytes.