A dual-promoter recombinant vector expressing an immunogenic protein of an African swine fever virus (ASFV), a recombinant bacterium, and use thereof are provided. A recombinant gene consisting of a PpepN promoter, a coding sequence of a Usp45 secretion signal peptide, and a coding sequence of the immunogenic protein of the ASFV that are ligated in sequence is inserted downstream of a promoter in an original vector to obtain the dual-promoter recombinant vector expressing the immunogenic protein of the ASFV.

Provided is an African swine fever virus chimeric protein. The chimeric protein comprises: (1) an African swine fever virus p72 domain I; (2) an African swine fever virus p72 domain II; (3) an African swine fever virus p72 domain III; and (4) an African swine fever virus antigenic protein. By using African swine fever virus p72 protein as a skeleton, the chimeric protein provided in the present invention will exhibit antigenic epitopes of African swine fever virus antigenic proteins p54, p30, CD2v, and p12, achieve a good immune effect, and can produce significant humoral and cell-mediated immune response.

A method for generating progeny of an African swine fever (ASF) virus includes providing an isolated or purified fetal porcine lung alveolar macrophage cell capable of replicating the ASF virus, wherein the cell is cultured for at least 5 passages; exposing the cell to the ASF virus; and allowing the ASF virus to replicate in the cell; thereby generating progeny of the ASF virus.