The invention relates to a process for the production of ethanol, the process comprising fermenting of a carbon source composition with a recombinant yeast,

wherein the carbon source composition comprises at least glucose and arabinose; and
wherein the recombinant yeast comprises arabinose isomerase activity, ribulokinase activity, ribulose phosphate epimerase activity, glycerol uptake activity and glycerol conversion capacity; and
wherein the recombinant yeast further comprises a genetic modification leading to the reduction, downregulation, inhibition and/or elimination of the activity of a homologous protein with glycerol-efflux activity; and
wherein each of the glucose and the arabinose is converted into ethanol.

In addition, the invention relates to a recombinant yeast that can be used in such a process.

The invention relates to a process for the production of ethanol, the process comprising fermenting of a carbon source composition with a recombinant yeast,

wherein the carbon source composition comprises at least glucose and arabinose; and
wherein the recombinant yeast comprises arabinose isomerase activity, ribulokinase activity, ribulose phosphate epimerase activity, glycerol uptake activity and glycerol conversion capacity; and
wherein the recombinant yeast further comprises a genetic modification leading to the reduction, downregulation, inhibition and/or elimination of the activity of a homologous protein with glycerol-efflux activity; and
wherein each of the glucose and the arabinose is converted into ethanol.

In addition, the invention relates to a recombinant yeast that can be used in such a process.

The present disclosure provides recombinant microorganisms and methods for producing malonate semialdehyde and/or related products, such as ketones, alcohols, organic acids, esters, alkenes, amino acids, and combinations thereof including 3-hydroxypropionic acid, acrylic acid, propionic acid, 1-propanol, acetone, 2-propanol, butanone, 1-butanol, 2-butanol, methyl propionate, 1,3-propanediol, isoamyl alcohol, 1,3-butanediol, 1,4-butanediol, 2,3-butanediol, lactic acid, adipic acid, glutamic acid, itaconic acid, ethyl acetate, isopropyl acetate, acetic acid, butyric acid, caproic acid, citric acid, methacrylic acid, succinic acid, propylene, butadiene, ethanol, isoprenol, leucine, isoleucine, glutamine, glycine, and isoprene, from β-alanine. The recombinant microorganism expresses an asparate 1-decarboxylase that catalyzes the production of malonate semialdehyde from β-alanine.

The present invention provides an isolated AZF1 gene sequence, recombinant vectors, and recombinant yeast which are useful in methods of enhanced biofuel production, particularly ethanol production. Methods of bioengineering recombinant yeast with isolated AZF1 gene sequence useful for biofuel production are also provided.

The present invention provides an isolated AZF1 gene sequence, recombinant vectors, and recombinant yeast which are useful in methods of enhanced biofuel production, particularly ethanol production. Methods of bioengineering recombinant yeast with isolated AZF1 gene sequence useful for biofuel production are also provided.

An improved dry grind system and method for producing a sugar stream from grains or similar carbohydrate sources and/or residues, such as for biochemical production. In particular, after saccharification and prior to a sugar conversion process, a sugar/carbohydrate stream is removed from a saccharified stream. The sugar/carbohydrate stream includes a desired Dextrose Equivalent (DE) where DE describes the degree of conversion of starch to dextrose can be produced, with the such sugar stream being available for biochemical production, e.g., alcohol production, or other processes. In addition, the systems and methods also can involve the removal of certain grain components, e.g., corn kernel components, including protein and/or fiber. Sugar stream production occurs on the front end of the system and method.

An improved dry grind system and method for producing a sugar stream from grains or similar carbohydrate sources and/or residues, such as for biochemical production. In particular, after saccharification and prior to a sugar conversion process, a sugar/carbohydrate stream is removed from a saccharified stream. The sugar/carbohydrate stream includes a desired Dextrose Equivalent (DE) where DE describes the degree of conversion of starch to dextrose can be produced, with the such sugar stream being available for biochemical production, e.g., alcohol production, or other processes. In addition, the systems and methods also can involve the removal of certain grain components, e.g., corn kernel components, including protein and/or fiber. Sugar stream production occurs on the front end of the system and method.

What is described is an integrated steel mill and a bioreactor configured to produce useful products from the waste stream of the steel mill. A waste gas stack which is connected to the steel mill is connected to a heat exchanger to cool the waste gas from the steel mill. The cooled gas is pressurized using a pressurization apparatus connected to the heat exchanger. The pressurized gas is sent to an oxygen removal apparatus connected to the pressurization apparatus. An oxygen depleted waste stream from the oxygen removal apparatus is passed to a bioreactor (connected to the oxygen removal apparatus) where microorganisms ferment the waste stream to products. Optional apparatus such as scrubbers, valves, buffers, are also disclosed. The products of the fermentation in the bioreactor can be ethanol and or acetate.

A method of obtaining a compound may include adding a substrate to a medium in a reactor, and reacting the substrate in the reactor to form the compound. A first stream is separated from the reaction liquid through a first membrane. A second stream is separated from the reaction liquid through a second membrane. The first membrane is a filtration membrane and the second membrane is configured for liquid-gas or liquid-liquid extraction The first membrane and the second membrane are at least partially immersed in the medium and are moved relative to the reactor during the separation steps.

A process for culturing Clostridium saccharoperbutylacetonicum cells, which are capable of growing on gamma-cyclodextrin in a liquid culture medium in a culture vessel. Also disclosed is a process for producing a bio-product, the process comprising culturing Clostridium saccharoperbutylacetonicum cells, which are capable of growing on gamma-cyclodextrin in a liquid culture medium in a culture vessel.