Provided herein are methods, compositions, and non-naturally occurring microbial organism for preparing compounds such as α-butanol, butyric acid, succinic acid, 1,4-butanediol, 1-pentanol, pentanoic acid, glutaric acid, 1,5-pentanediol, 1-hexanol, hexanoic acid, adipic acid, 1,6-hexanediol, 6-hydroxy hexanoic acid, ε-Caprolactone, 6-amino-hexanoic acid, ε-Caprolactam, hexamethylenediamine, linear fatty acids and linear fatty alcohols that are between 7-25 carbons long, linear alkanes and linear α-alkenes that are between 6-24 carbons long, sebacic acid and dodecanedioic acid comprising: a) converting a C.sub.N aldehyde and pyruvate to a C.sub.N+3 β-hydroxyketone intermediate through an aldol addition; and b) converting the C.sub.N+3 β-hydroxyketone intermediate to the compounds through enzymatic steps, or a combination of enzymatic and chemical steps.

[Problem] It is an object of the present invention to provide a method for collecting seawater that contains plankton and producing DHMBA, which is an antioxidant, from the plankton contained in the seawater.

[Solution] The method of the present invention includes: filtering collected seawater containing the plankton using a filter; taking out a cell content from the plankton remaining on the filter; subsequently heating/pressurizing the cell content taken out; and producing 3,5-dihydroxy-4-miethoxybenzyl alcohol from the heated/pressurized product. The plankton is a diatom. [Selected Drawing] FIG. 1

[Problem] It is an object of the present invention to provide a method for collecting seawater that contains plankton and producing DHMBA, which is an antioxidant, from the plankton contained in the seawater.

[Solution] The method of the present invention includes: filtering collected seawater containing the plankton using a filter; taking out a cell content from the plankton remaining on the filter; subsequently heating/pressurizing the cell content taken out; and producing 3,5-dihydroxy-4-miethoxybenzyl alcohol from the heated/pressurized product. The plankton is a diatom. [Selected Drawing] FIG. 1

Provided is a method which enables efficient separation of a component such as microorganisms from an organic substance-containing liquid obtained by microbial fermentation. Disclosed is a method for producing an organic substance comprising a microbial fermentation step of obtaining an organic substance-containing liquid and a separation step of heating the organic substance-containing liquid and separating into a liquid or solid component containing microorganisms and a gaseous component containing the organic substance.

Among other things, the present disclosure provides biosynthesis polypeptides, methods, and non-naturally occurring microbial organisms for preparing various compounds such as 1,5-pentanediol, adipic acid, 1,6-hexanediol, 6-hydroxy hexanoic acid, and 2-keto carboxylic acids.

This document describes biochemical pathways for producing 7-aminoheptanoic acid using a β-ketoacyl synthase or a β-ketothiolase to form an N-acetyl-5-amino-3-oxopentanoyl-CoA intermediate. 7-aminoheptanoic acid can be enzymatically converted to pimelic acid, 7-hydroxyheptanoic acid, heptamethylenediamine or 1,7-heptanediol or corresponding salts thereof. This document also describes recombinant microorganisms producing 7-aminoheptanoic acid as well as pimelic acid, 7-hydroxyheptanoic acid, heptamethylenediamine and 1,7-heptanediol or corresponding salts thereof.

This document describes biochemical pathways for producing 7-aminoheptanoic acid using a β-ketoacyl synthase or a β-ketothiolase to form an N-acetyl-5-amino-3-oxopentanoyl-CoA intermediate. 7-aminoheptanoic acid can be enzymatically converted to pimelic acid, 7-hydroxyheptanoic acid, heptamethylenediamine or 1,7-heptanediol or corresponding salts thereof. This document also describes recombinant microorganisms producing 7-aminoheptanoic acid as well as pimelic acid, 7-hydroxyheptanoic acid, heptamethylenediamine and 1,7-heptanediol or corresponding salts thereof.

An object of the present invention is to provide a simple method of producing ergothioneine. The present invention provides a method of producing ergothioneine comprising a step of culturing a microbe belonging to the genus Moniliella in a medium containing a carbon source to allow the microbe to produce ergothioneine.

An object of the present invention is to provide a simple method of producing ergothioneine. The present invention provides a method of producing ergothioneine comprising a step of culturing a microbe belonging to the genus Moniliella in a medium containing a carbon source to allow the microbe to produce ergothioneine.

The present disclosure discloses recombinant Escherichia coli and application thereof in screening erythritol-producing strains, and belongs to the technical field of microorganisms. The recombinant Escherichia coli used in a method for screening an erythritol-producing strain disclosed by the present disclosure can well perform positive correlation induction on erythritol with different concentrations, so that the method for screening the erythritol-producing strain has the advantage of high sensitivity. High-concentration glucose is usually adopted as a fermentation substrate when erythritol is produced in a fermentation mode in the industry, but the method for screening the erythritol-producing strain disclosed by the present disclosure can overcome the interference of the high-concentration glucose, and under the interference of the high-concentration glucose, the recombinant Escherichia coli used in the method for screening the erythritol-producing strain can still well perform positive correlation induction on erythritol with different concentrations, and the correlation is higher than that without the interference of the glucose. Therefore, the method for screening the erythritol-producing strain has the advantage of strong anti-interference capability.