The invention discloses a method for improving citric acid production by Aspergillus niger fermentation, which integrates Aspergillus niger GABA pathway succinate semialdehyde dehydrogenase SSD gene into Aspergillus niger genome to obtain recombinant Aspergillus niger strain, and uses recombinant black The Aspergillus strain ferments to produce citric acid; the expression of the succinate semialdehyde dehydrogenase SSD gene is regulated by the Pgas promoter. The method of the invention realizes the expression of succinate semialdehyde dehydrogenase SSD in Aspergillus niger to enhance the GABA pathway so as to strengthen the TCA cycle and promote the synthesis of citric acid.

The present disclosure relates to a genetically modified microbial cell that includes a genetic modification resulting in the expression of a vanillate demethylase, where the microbial cell is capable of metabolizing at least one S-lignin decomposition molecule including at least one of syringate and/or 3-O-methyl gallate, and the genetically modified microbial cell is capable of producing gallate. In some embodiments of the present disclosure, the vanillate demethylase may include VanAB.

The present invention relates to glucoamylase variants having improved thermostability. The present invention also relates to polynucleotides encoding the variants; nucleic acid constructs, vectors, and host cells comprising the polynucleotides; and methods of using the variants.

The present invention relates to glucoamylase variants having improved thermostability. The present invention also relates to polynucleotides encoding the variants; nucleic acid constructs, vectors, and host cells comprising the polynucleotides; and methods of using the variants.

Biomass (e.g., plant biomass, animal biomass, and municipal waste biomass) is processed to produce useful intermediates and products, such as hydroxy-carboxylic acids and hydroxy-carboxylic acid derivatives. A method includes treating a reduced recalcitrance lignocellulosic or cellulosic material with one or more enzymes and/or organisms (such as lactobacillus, Pediococcus, Rhizopus, Enterococcus) to produce an alpha, beta, gamma and/or delta hydroxycarboxylic acid (such as lactic acid, glycolic acid); and converting the alpha, beta, gamma and/or delta hydroxy-carboxylic acid to the product (such as esters, polymers, and copolymers).

Biomass (e.g., plant biomass, animal biomass, and municipal waste biomass) is processed to produce useful intermediates and products, such as hydroxy-carboxylic acids and hydroxy-carboxylic acid derivatives. A method includes treating a reduced recalcitrance lignocellulosic or cellulosic material with one or more enzymes and/or organisms (such as lactobacillus, Pediococcus, Rhizopus, Enterococcus) to produce an alpha, beta, gamma and/or delta hydroxycarboxylic acid (such as lactic acid, glycolic acid); and converting the alpha, beta, gamma and/or delta hydroxy-carboxylic acid to the product (such as esters, polymers, and copolymers).

The present invention relates to a process for the production of an organic acid from a lignocellulosic feedstock. The process is integrated with a pulp mill and comprises the steps: a) providing a lignocellulosic feedstock; b) obtaining an alkaline liquor from the pulp mill; c) pre-treating the lignocellulosic feedstock with the alkaline liquor, thereby obtaining a pretreated cellulosic feed and a black liquor; d) obtaining calcium oxide from the pulp mill; e) subjecting the pretreated cellulosic feed from step c) to enzymatic hydrolysis, thereby obtaining a saccharide feed; f) subjecting the saccharide feed from step e) to microbial fermentation using the calcium oxide from step d) as a neutralising agent, thereby obtaining an organic acid calcium salt; g) treating the organic acid calcium salt with sulfuric acid, thereby obtaining gypsum and the organic acid; h) optionally isolating lignin from the black liquor obtained in step c), thereby obtaining lignin and weak black liquor; and i) returning the black liquor obtained in step c) and/or the weak black liquor obtained in step h) to the pulp mill for integration with the pulp mill chemical recovery process; wherein steps e) and f) are performed either sequentially or simultaneously.

The present invention relates to a process for the production of an organic acid from a lignocellulosic feedstock. The process is integrated with a pulp mill and comprises the steps: a) providing a lignocellulosic feedstock; b) obtaining an alkaline liquor from the pulp mill; c) pre-treating the lignocellulosic feedstock with the alkaline liquor, thereby obtaining a pretreated cellulosic feed and a black liquor; d) obtaining calcium oxide from the pulp mill; e) subjecting the pretreated cellulosic feed from step c) to enzymatic hydrolysis, thereby obtaining a saccharide feed; f) subjecting the saccharide feed from step e) to microbial fermentation using the calcium oxide from step d) as a neutralising agent, thereby obtaining an organic acid calcium salt; g) treating the organic acid calcium salt with sulfuric acid, thereby obtaining gypsum and the organic acid; h) optionally isolating lignin from the black liquor obtained in step c), thereby obtaining lignin and weak black liquor; and i) returning the black liquor obtained in step c) and/or the weak black liquor obtained in step h) to the pulp mill for integration with the pulp mill chemical recovery process; wherein steps e) and f) are performed either sequentially or simultaneously.

Methods of redirecting carbon flux and increasing C2/C3 or a C4/5/6 carbon chain length carbon-based chemical product yield in an organism, nonnaturally occurring organisms with redirected carbon flux and increased C2/C3 or C4/5/6 carbon chain length carbon-based chemical product yield and methods for using these organisms in production of C2/C3 or C4/5/6 carbon chain length carbon-based chemical products are provided.

This disclosure provides a genetically-modified bacterium from the genus Pseudomonas that produces itaconate or trans-aconitate. The disclosure further provides methods for producing itaconate or trans-aconitate using a genetically-modified bacterium from the genus Pseudomonas.