Described herein are genetically-modified microorganisms for producing long-chain dicarboxylic acids and methods of using the microorganisms. The microorganisms contain a first nucleic acid encoding an Umbellularia californica lauroyl ACP-thioesterase (BTE) operably linked to a promoter or a second nucleic acid encoding a Cocos nucifera lauroyl ACP-thioesterase (FatB3) operably linked to a promoter.

A method and system for efficiently producing a fermentative product alcohol such as butanol utilizing in situ product extraction are provided. The efficiency is obtained through separating undissolved solids after liquefying a given feedstock to create a feedstock and prior to fermentation, for example, through centrifugation. Removal of the undissolved solids avoids problems associated with having the undissolved solids present during in situ production extraction, and thereby increases the efficiency of the alcohol production.

The present invention relates to a lipase variant of a parent lipase, which variant has lipase activity, at least 75% but less than 100% sequence identity to SEQ ID NO: 3 and comprises a substitution at one or more positions corresponding to positions 1; 2; 3; 4; 5; 6; 7; 9; 10; 11; 12; 16; 19; 30; 31; 34; 36; 37; 39; 40; 42; 44; 51; 52; 53; 54; 56; 58; 59; 70; 71; 72; 73; 83; 84; 86; 88; 90; 92; 93; 95; 96; 100; 101; 102; 104; 106; 109; 110; 112; 117; 119; 124; 125; 127; 128; 131; 132; 133; 134; 135; 137; 158; 159; 160; 161; 162; 163; 165; 166; 167; 168; 170; 181; 182; 183; 189; 190; 192; 194; 196; 202; 210; 211; 212; 220; 225; 227; 228; 229; 230; 231; 233; 237; 238; 239; 240; 242; 246; 247; 248; 252; 259; 262; 264; 269 of SEQ ID NO: 3. The present invention also relates to polynucleotides encoding the variants; nucleic acid constructs, vectors, and host cells comprising the polynucleotides; and methods of using the variants.

The disclosure relates to the field of specialty chemicals and methods for their synthesis. In embodiments, the disclosure provides viable bacterial cells which comprise heterologous dual 3-hydroxy-acyl-ACP dehydratase/isomerases, etc. The disclosure further provides monounsaturated fatty acid derivative molecules produced by the viable bacterial cells which are non-native to the bacterial cells. The disclosure further provides methods for the preparation and production of non-native monounsaturated fatty acid derivative molecules such as e.g., an 3-monounsaturated fatty acid derivative, an 5-monounsaturated fatty acid derivative, an 9-monounsaturated fatty acid derivative, an 11-monounsaturated fatty acid fatty acid derivative, etc.

The disclosure relates to the field of specialty chemicals and methods for their synthesis. In embodiments, the disclosure provides viable bacterial cells which comprise heterologous dual 3-hydroxy-acyl-ACP dehydratase/isomerases, etc. The disclosure further provides monounsaturated fatty acid derivative molecules produced by the viable bacterial cells which are non-native to the bacterial cells. The disclosure further provides methods for the preparation and production of non-native monounsaturated fatty acid derivative molecules such as e.g., an 3-monounsaturated fatty acid derivative, an 5-monounsaturated fatty acid derivative, an 9-monounsaturated fatty acid derivative, an 11-monounsaturated fatty acid fatty acid derivative, etc.

Provided here is an enzymatic process for production of low saturate oil, in one embodiment, low palmitic oils from triacylglycerol sources. The enzymes used in the processes herein are saturase enzymes, including palmitase enzymes. The oils produced by the processes herein are used in food products.

[Problems] To provide a method of producing a medium chain fatty acid or a lipid containing this fatty acid as a component by using a -ketoacyl-ACP synthase, and a gene, a protein and a transformant for use in this method.

[Means to solve] A method of producing a medium chain fatty acid or a lipid containing this fatty acid as a component, containing the steps of: introducing a gene encoding the following protein (A) or (B) into a host, and thereby obtaining a transformant, and collecting a medium chain fatty acid or a lipid containing this fatty acid as a component from the resulting transformant; and a gene, a protein and a transformant for use in this method:
(A) A protein consisting of the amino acid sequence set forth in SEQ ID NO: 1; and
(B) A protein consisting of an amino acid sequence having 90% or more identity with the amino acid sequence set forth in SEQ ID NO: 1, and having medium chain acyl-ACP-specific -ketoacyl-ACP synthase activity.

Disclosed herein are methods of manufacturing renewable chemicals through the manufacture of novel triglyceride oils followed by chemical modification of the oils. Methods such as transesterification, hydrogenation, hydrocracking, deoxygenation, isomerization, interesterification, hydroxylation, hydrolysis and saponification are disclosed. Novel oils containing fatty acid chain lengths of C8, C10, C12 or C14 are also disclosed and are useful as feedstocks in the methods of the invention.

The present invention relates to compositions comprising polypeptides having phospholipase C activity and to the use of such compositions for hydrolysing a phospholipid or lysophospholipid, The invention also relates to processes for hydrolysing a phospholipid or lysophospholipid, comprising contacting said phospholipid or lysophospholipid with a composition or a pluralities of polypeptides having Phospholipase C activity according to the invention.

The present invention relates to lipid compositions derived from insects. The intrinsic chemical/physical properties of insect derived lipids limit the possibilities for widespread and large-scale use. An enzymatic process for the partial hydrolysis and transesterification of insect extracted lipids is provided. The process alters the ratio of triglyceride (TAG), diglycerides (DAG), monoglycerides (MAG) and free fatty acids (FFA) of the crude insect lipid and also results in the transesterification of fatty acids on glyceride backbone yielding a lipid composition that combines an increased dispersibility in water, a more favourable hardness profile (as a function of temperature) and a melting point slightly above ambient temperature. The compositions of the invention further have good nutritive properties as well as some interesting functional properties, e.g. anti-microbial effects. The invention relates to these enzyme-modified lipid compositions, to the methods for producing them, as well as to their uses as an ingredient in an alimentary product.