The present invention provides a method for extracting lipids from microorganisms without using organic solvent as an extraction solvent. In particular, the present invention provides a method for extracting lipids from microorganisms by lysing cells and removing water soluble compound and/or materials by washing the lysed cell mixtures with aqueous washing solutions until a substantially non-emulsified lipid is obtained.

The invention relates to a method for enriching protists with lipids rich in polyunsaturated fatty acids (PUFA) in particular of the omega 3 (ω3) class, said method comprising the culture of protists in a culture medium comprising a selenium-containing compound as well as the protists enriched with lipids rich in PUFA obtained by this method. The invention also relates to a method for producing lipids rich in PUFA, comprising, after the enrichment method according to the invention, a method for treating the protists by extraction from the biomass of selenium-containing lipids rich in PUFA. The invention finally relates to any food, cosmetic or pharmaceutical product comprising either selenium-containing lipids thus extracted, or selenium-containing biomass originating from the enrichment method.

The invention relates to a method for enriching protists with lipids rich in polyunsaturated fatty acids (PUFA) in particular of the omega 3 (ω3) class, said method comprising the culture of protists in a culture medium comprising a selenium-containing compound as well as the protists enriched with lipids rich in PUFA obtained by this method. The invention also relates to a method for producing lipids rich in PUFA, comprising, after the enrichment method according to the invention, a method for treating the protists by extraction from the biomass of selenium-containing lipids rich in PUFA. The invention finally relates to any food, cosmetic or pharmaceutical product comprising either selenium-containing lipids thus extracted, or selenium-containing biomass originating from the enrichment method.

The present disclosure provides methods and materials for the cultivation and/or propagation of a photosynthetic organism. Such methods may comprise the use of a lamp assembly that comprises a plurality of circuit boards, each comprising at least three edges, arranged in a substantially spherical shape defining an interior lamp assembly volume, wherein the plurality of circuit boards comprise a first planar surface in contact with the interior lamp assembly volume and an opposing second planar surface comprising light emitting diodes (LEDs); and a barrier that surrounds the plurality of circuit boards forming the substantially spherical shape.

This document describes biochemical pathways for producing pimeloyl-CoA using a polypeptide having the enzymatic activity of a hydroperoxide lyase to form non-3-enal and 9-oxononanoate from 9-hydroxyperoxyoctadec-10,12-dienoate. Non-3-enal and 9-oxononanoate can be enzymatically converted to pimeloyl-CoA or a salt thereof using one or more polypeptides having the activity of a dehydrogenase, a CoA ligase, an isomerase, a reductase, a thioesterase, a monooxygenase, a hydratase, and/or a thiolase. Pimeloyl-CoA can be enzymatically converted to pimelic acid, 7-aminoheptanoic acid, 7-hydroxyheptanoic acid, heptamethylenediamine, or 1,7-heptanediol, or corresponding salts thereof. This document also describes recombinant microorganisms producing pimeloyl-CoA, as well as pimelic acid, 7-aminoheptanoic acid, 7-hydroxyheptanoic acid, heptamethylenediamine, and 1,7-heptanediol, or corresponding salts thereof.

Disclosed are compositions and methods related to eukaryotic microorganisms that can produce unsaturated fatty acids which can be purified and used.

The present invention provides a production method of oxo fatty acid, as well as rare fatty acids such as conjugated fatty acid, hydroxylated fatty acid, partially saturated fatty acid and the like, which uses 4 kinds of enzymes (fatty acid-hydratase, hydroxylated fatty acid-dehydrogenase, oxo fatty acid-isomerase, oxo fatty acid-enone reductase) derived from Lactobacillus plantarum including lactic acid bacteria and the like. Furthermore, the present invention also provides a more efficient production method of oxo fatty acid and the like, which partly uses a chemical oxidation reaction in combination.

The invention concerns a strain of Schizochytrium mangrovei, filed on 22 Nov. 2012 with the CNCM as number I-4702, having the ability to produce a high quantity of docosahexaenoic acid (or DHA) and palmitic acid, the methods for producing the corresponding biomass containing said lipid compounds of interest, and the biomass containing the products and compositions prepared from this strain.

Disclosed is a method for synthesis of fatty acids by culturing a eukaryotic microorganism from the fungi kingdom, that is naturally oleaginous or rendered oleaginous. The culture is performed in the presence a fatty acid synthase inhibitor in the culture medium.

The invention concerns a method of sustainably producing an aquaculture meat product by feeding a fish over its dietary cycles an aquaculture feed composition, said method comprising the step of formulating an aquaculture feed composition by replacing all or part of fish oil in the composition with a single microbial source of eicosapentaenoic acid (“EPA”) and docosahexaenoic acid (“DHA”). In a preferred embodiment, the microbial source comprising DHA and EPA derives from a microorganism/microbe of the genus Schizochytrium or Thraustochytrium.