Disclosed herein are mutant photosynthetic microorgnaisms having an attenuated SGI1 gene. The mutants have reduced chlorophyll and increased productivity with respect to wild type cells. Also disclosed are methods of using such mutants for producing biomass or bioproducts, and methods of screening for such mutants.

Disclosed herein are mutant photosynthetic microorgnaisms having an attenuated SGI1 gene. The mutants have reduced chlorophyll and increased productivity with respect to wild type cells. Also disclosed are methods of using such mutants for producing biomass or bioproducts, and methods of screening for such mutants.

The invention describes carbon fibers which are produced on the basis of different process chains from CO2. These include routes through natural resources such as algal biomass to produce carbon fiber precursors such as PAN from CO2, as well as the purely synthetic route via the Fischer-Tropsch synthesis, which is also used to make CO2 carbon fiber precursors. In this way, CO2 from anthropogenic origin is to be converted into a solid aggregate state of carbon fiber, which can be disposed of at the end of its life cycle, after being used as highly valuable building material for industry and man, for the construction of buildings and vehicles. These processes produce by-products such as biodiesel and nutrients that generate added value. The production volumes of the resulting substances should be controllable by combining the methods presented here. Some of these processes alone have no long-term climate relevance because of the high costs, but in the initial phase of such a development with the help of carbon dioxide certificates or socio-political necessities they are able to quickly show that carbon fiber building materials can be produced which by themselves are made from CO2 and at least have the quality to be used in the construction sector and for example are feasible to replace steel, in that the paradigm of todays material production being CO2-positive, can be turned into the opposite. If the processeswhich have the disadvantage of large-area consumption on the one hand and the of the lack of energy efficiency in the longer term on the othercan be coupled, they have the potential to support each other. By combining the methods, land use and costs can be adjusted to current regional economic performance based on the material paradigm of the future of carbon-negative production of carbon fibers, also depending on the current evolution of CO2 emission allowance prices. The invention has the desired effect in climate policy that high-tech technology transfer can take place into the currently disadvantaged regions of the world, which promotes the economic performance of today's disadvantaged regions and in particular creates the urgently needed jobs in these regions.

The present invention generally related to a fungal cell capable of tailored triacylglycerols. The fungal cell comprises at least one modification to the endogenous fatty acid metabolism.

A method of producing lipids, containing the steps of: culturing a transformant in which the expression of a gene encoding the following protein (A) or (B) is enhanced, and producing fatty acids or lipids containing these fatty acids as components: (A) a protein consisting of the amino acid sequence set forth in SEQ ID NO: 1; and (B) a protein consisting of an amino acid sequence having 44% or more identity with the amino acid sequence of the protein (A), and having acyltransferase activity.

The disclosed subject matter relates generally to a method for modifying oil, and specifically to a process for increasing the concentration of polyunsaturated fatty acid in an oil composition.

The present invention relates to a polypeptide having phospholipase C activity, selected from the group consisting of i. a polypeptide comprising a mature polypeptide sequence of SEQ ID NO: 2; ii. a polypeptide that has least 85%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98% or 99% identity to the mature polypeptide sequence of SEQ ID NO: 2; iii. a polypeptide encoded by a nucleic acid that hybridizes under medium stringency, preferably under high stringency conditions to the complementary strand of the mature polypeptide coding sequence of SEQ ID NO:1; iv. a polypeptide encoded by a nucleic acid that has at least 85%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99% or 100% identity to the mature polypeptide coding sequence of SEQ ID NO: 1. A process for degumming a vegetable oil comprising contacting a vegetable oil comprising phospholipids with a polypeptide having phospholipase C activity of the invention or a composition of the invention, wherein phospholipids are hydrolyzed into diacylglycerol and phosphate ester and/or phosphate, separating the phosphate ester and/or phosphate from the vegetable oil wherein a degummed vegetable oil is obtained.

DGA1 catalyzes the final enzymatic step for converting acyl-CoA and 1,2-diacylglycerol to triacylglycerols (TAG) and CoA in yeast. Disclosed are methods for expression in an oleaginous yeast host of polynucleotide sequences encoding DGA1 from Rhodosporidium toruloides, Lipomyces starkeyi, Aurantiochytrium limacinum, Aspergillus terreus, or Claviceps purpurea. Also described herein are engineered recombinant host cells of Yarrowia lipolytica comprising heterologous DGA1 polynucleotides encoding DGA1 proteins, or functionally active portions thereof, having the capability of producing increased lipid production and possessing the characteristic of enhanced glucose consumption efficiency.

The present invention relates to a method of reducing the phospholipid content in an oil or fat composition and polypeptides having PI-specific phospholipase C activity as well as polypeptides having PC, PE-specific phospholipase C activity and combinations thereof capable of catalyzing this reduction. The invention also relates to polynucleotides encoding the polypeptides, nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.

The disclosed subject matter relates generally to a method for modifying oil, and specifically to a process for increasing the concentration of polyunsaturated fatty acid in an oil composition.