The present invention relates to a method for detecting a target nucleic acid, which induces any surrogate target to be amplified in the presence of the target nucleic acid and is useful for molecular diagnosis, prenatal diagnosis, early diagnosis, cancer diagnosis, genetic related diagnosis, genetic trait diagnosis, diagnosis of infectious bacteria, identification of drug-resistant bacteria, forensic medicine, species identification of organisms, and the like.

Provided herein are oligonucleotide probes for detecting 2019 novel coronavirus (2019-nCoV). The probes are modified at their 5′ ends with a fluorophore (e.g., fluorescein), and are also modified (e.g., at their 3′ ends) with a moiety capable of quenching fluorescence from the fluorophore. The moiety is based on the IQ-4 or IQ-2 quencher. Also provided are kits including one or more of such oligonucleotide probes, and methods of detecting 2019-nCoV and/or diagnosing COVID-19 using the oligonucleotide probes and kits described herein.

Disclosed is a probe for use in biological assays. The probe includes a fluorescent dye bound to a quencher compound through an oligonucleotide linker. Also disclosed are methods of using the probe, such as for a polymerase chain reaction (PCR), such as in a quantitative PCR reaction (qPCR), as well as kits including the probe.

Disclosed is a probe for use in biological assays. The probe includes a fluorescent dye bound to a quencher compound through an oligonucleotide linker. Also disclosed are methods of using the probe, such as for a polymerase chain reaction (PCR), such as in a quantitative PCR reaction (qPCR), as well as kits including the probe.

Methods for identifying co-occurrence of nucleic acid segments in a nucleic acid sample from a specimen including obtaining a nucleic acid sample from a specimen, determining sequences of first and second nucleic acid segments in nucleic acid fragments of the sample to generate a first and second sets of sequences, generating a first and second sets of probes from the first and second sets of sequences, exposing a detection sample to a member of the first set of probes and a member of the second set of probes, performing a hybridization analysis to determine whether the members of the first and second sets of probes hybridize to the detection sample, and determining whether the first and second nucleic acid segments co-occur in a common cell of the specimen.

Disclosed herein are mechanically-strained oligonucleotide constructs comprising two oligonucleotides that when hybridized results in a bent double-stranded oligonucleotide. The constructs may be used to probe oligonucleotide interactions with an analyte to detect interactions with metal ions or compounds.

Disclosed herein are mechanically-strained oligonucleotide constructs comprising two oligonucleotides that when hybridized results in a bent double-stranded oligonucleotide. The constructs may be used to probe oligonucleotide interactions with an analyte to detect interactions with metal ions or compounds.

Methods and compositions for attaching cell-specific barcodes without formation of partitions is provided.

This invention relates to methods for analyzing nucleic acids. The disclosure provides methods of a primer-dependent amplification and detection method that is capable of amplifying and detecting in a sample as few as ten copies of at least one rare intended target sequence in the presence of abundant closely related unintended target sequences. Also provided are reaction compositions and kits for performing the methods.

This invention relates to methods for analyzing nucleic acids. The disclosure provides methods of a primer-dependent amplification and detection method that is capable of amplifying and detecting in a sample as few as ten copies of at least one rare intended target sequence in the presence of abundant closely related unintended target sequences. Also provided are reaction compositions and kits for performing the methods.