Methods for determining locations of analytes include: (a) exposing a biological sample to a plurality of different types of probes, where each different type of probe includes a nucleic acid capture moiety and a detection moiety that includes at least one reporter moiety, where the at least one reporter moiety features multiple label regions, each of the label regions including an oligonucleotide having a sequence; (b) exposing the biological sample to a plurality of optical labels; (c) measuring optical signals generated by optical labels; (d) repeating steps (b) and (c) with different pluralities of optical labels; (e) identifying one or more of the reporter moieties in the sample based on the measured optical signals; and (f) determining a location of one or more of the RNA analytes in the sample based on the identified reporter moieties.

Methods for determining locations of analytes include: (a) exposing a biological sample to a plurality of different types of probes, where each different type of probe includes a nucleic acid capture moiety and a detection moiety that includes at least one reporter moiety, where the at least one reporter moiety features multiple label regions, each of the label regions including an oligonucleotide having a sequence; (b) exposing the biological sample to a plurality of optical labels; (c) measuring optical signals generated by optical labels; (d) repeating steps (b) and (c) with different pluralities of optical labels; (e) identifying one or more of the reporter moieties in the sample based on the measured optical signals; and (f) determining a location of one or more of the RNA analytes in the sample based on the identified reporter moieties.

The present invention is directed to assay methods and compounds and kits used in such assays.

The present invention is directed to assay methods and compounds and kits used in such assays.

The present disclosure provided methods and compositions for nucleic acid sequencing. In particular, the disclosure provides for detection of multiple different nucleotides in a sample utilizing fewer detection moieties than the number of nucleotides being detected and using two imaging events per sequencing cycle.

The present disclosure provided methods and compositions for nucleic acid sequencing. In particular, the disclosure provides for detection of multiple different nucleotides in a sample utilizing fewer detection moieties than the number of nucleotides being detected and using two imaging events per sequencing cycle.

The present application relates to detection of changes in the number of nucleotides in short homopolymeric nucleic acid repeats, in particular in short homopolymeric microsatellites, for example for the purpose of diagnosing microsatellite instability (MSI) and/or mismatch repair (MMR-) deficiency in tumors. Accordingly, methods are provided for detecting changes in the number of nucleotides present in short homopolymeric nucleotide repeat sequences as well as kits and cartridges for automated detection of said changes.

The present application relates to detection of changes in the number of nucleotides in short homopolymeric nucleic acid repeats, in particular in short homopolymeric microsatellites, for example for the purpose of diagnosing microsatellite instability (MSI) and/or mismatch repair (MMR-) deficiency in tumors. Accordingly, methods are provided for detecting changes in the number of nucleotides present in short homopolymeric nucleotide repeat sequences as well as kits and cartridges for automated detection of said changes.

Provided are methods and devices for single-molecule genomic analysis. In one embodiment, the methods entail processing a double-stranded nucleic acid and characterizing said nucleic acid. These methods are useful in, e.g. determining structural variations and copy number variations between individuals.

Provided are methods and devices for single-molecule genomic analysis. In one embodiment, the methods entail processing a double-stranded nucleic acid and characterizing said nucleic acid. These methods are useful in, e.g. determining structural variations and copy number variations between individuals.