The present disclosure relates to the isolation, identification and characterization of novel sporeforming probiotic strain(s) with NSPase (Non-Starch Polysaccharides-active hydrolases) activity isolated from fish gut microbiota, methods and uses thereof. The sporeforming probiotic strain(s) with NSPase activity now disclosed are able of producing carbohydrate-active enzymes (CAZymes) that hydrolyse non-starch polysaccharides (NSPs) and accesses their potential as probiotics (PRO) for use in particular in aquafeeds.

The present invention provides a consortium of resistant microorganisms for biodegrading carbohydrates in the organic fraction of any source of solid waste. The genetic, metabolic and morphological structure of these resistant microorganisms works efficiently by biodegrading and mineralising solid biodegradable waste from municipal or harvest solid waste, reducing the production of gases and leachates. This compound enriches and increases the concentration of beneficial microorganisms, generating high-quality biological fertilisers suitable for use in agricultural production, in land recovery and conservation, under the parameters established in sustainable organic farming, which seeks to conserve, recover and use nature or the environment without generating the least negative impact.

The present invention relates to a feed additive comprising a Bacillus subtilis strain and a Bacillus licheniformis strain, a feed composition comprising the feed additive, and a method for producing the feed additive.

The present invention relates to an animal feed or an animal feed additive comprising Bacillus strains which improve the health and performance of production animals, and the use of such.

The present invention relates in general to bacterial cells having a genetic alteration that results in increased expression of a protein of interest and methods of making and using such cells. Aspects of the present invention include Grampositive microorganisms, such as members of the Bacillus genus having a genetic alteration that delays, reduces, or blocks the expression or activation of genes for sporulation, thereby resulting in enhanced expression of a protein of interest. The genetic alteration is one that reduces expression of a kinA gene, a phrA gene or a phrE gene.

The present invention relates to a bio-assisted method for treatment of spent caustic by treating with haloalkaliphilic consortium of bacteria capable of reducing or transforming sulphides, thiols, mercaptants and other sulphur containing compounds, phenols, hydrocarbons, naphthenic acids and their derivatives in spent caustic.

The present invention relates to methods of improving cell lysis procedures and yields of intracellular biomolecules extracted from biomass. The methods comprise storing the microbial cells in ultra-low temperature (ULT) conditions for at least 10 minutes prior to lysing the cells.

A Bacillus strain characterized by (i): sensitivity for ampicillin, vancomycin, gentamicin, kanamycin, streptomycin, erythromycin, clindamycin, tetracycline and chloramphenicol; (ii) antimicrobial activity against E. coli and Clostridium perfringens; and (iii) a sporulation percentage of at least 80 when measured after 2 days of incubation. The invention further relates to a method for selecting such strains. Many of the identified strains according to the invention are of the species Bacillus amyloliquefaciens. Some of the Bacillus amyloliquefaciens were further identified as Bacillus amyloliquefaciens subsp. amyloliquefaciens whereas others were identified as amyloliquefaciens subsp. plantarum. A Bacillus strain of the invention may be used as a feed additive to animal feed where it has a probiotic effect.

Compositions and methods are provided for producing silage additive composition for producing silage and for controlling pests that infect silage and stored grains. The silage additive composition comprises a microbial inoculant and diatomaceous earth, wherein the microbial inoculant comprises one or more strains of bacteria, wherein the at least one of one or more strains bacteria is capable of producing lactic acid.

The present invention refers to a process designed to increase the production of plant growth-promoting microorganisms of the Bacillus genus, using a culture medium poor in nutrients and with specific environmental conditions, allowing to obtain a greater amount of biomass and/or spores, which can be used to prepare solid or liquid compositions to be applied to plants, aiming to promote their growth and/or counteract the effect of phytopathogenic agents.