Method of extracting a partially purified extracellular metabolite composition from Bacillus coagulans MTCC 5856 and its antimicrobial effects thereof are described.

The present invention relates to a method for determining the methicillin-resistance properties of a Staphylococcus aureus strain present in a biological sample, the method comprising the following steps: a) incubating the biological sample containing the Staphylococcus aureus strain for at least 15 minutes in the presence of an antibiotic from the class of beta-lactams selected from the following group: cefoxitin and 6-APA (6-aminopenicillanic acid); b) isolating the bacteria present in the biological sample; c) lysing the bacteria and hydrolysing the bacterial proteins in order to obtain a mixture of peptides; and d) analysing this mixture of peptides by targeted mass spectrometry, the detection of at least one peptide originating from the protein PBP2a (SEQ ID NO. 1) or PBP2c (SEQ ID NO. 2) during this analysis step being indicative of the methicillin resistance of the Staphylococcus aureus strain present in the biological sample.

The present invention relates to methods and compositions for treatment of microbial infections and for the enhancement of resistance to infection. The invention comprises administration of an effective amount of bacterial lysate compositions for the treatment of pathological conditions of microbial infections. The present invention can also be used to enhance the immune system to prevent infections by the administration of an effective amount of the compositions.

The present invention relates to methods and compositions for treatment of microbial infections and for the enhancement of resistance to infection. The invention comprises administration of an effective amount of bacterial lysate compositions for the treatment of pathological conditions of microbial infections. The present invention can also be used to enhance the immune system to prevent infections by the administration of an effective amount of the compositions.

Disclosed are methods of treating and/or inhibiting an intramammary infection in a mammalian subject. The methods included administering a therapeutically effective amount of surface-associated polypeptides from various bacteria. Also disclosed are methods for blocking or reducing infection in a mammal. Further disclosed are methods for reducing the rate of intra-mammary infection and/or mastitis in a dairy mammal. Methods of increasing milk production are also provided.

Methods to edit genes by administering a chimeric nuclease to a cell or organism without the use of a viral vector.

The present invention provides a culture medium and use thereof in water toxicity colorimetric detection. The culture medium comprises glucose, peptone, NaCl, a beef extract, and a ferric salt. The solubility of the ferric salt in water is not high, such that part of the ferric salt remains resuspended in a solution along with cultured bacteria to continuously release Fe.sup.3+ after centrifugation and washing, thereby successfully constructing a water toxicity colorimetric detection sensor which is simple, environment-friendly, and visible to naked eyes.

The present invention relates to a method of treating organic waste comprising the steps of: treating the organic waste with a microorganism composition comprising the following microorganism species: Niallia sp., Stenotrophomonas sp. and Staphylococcus sp., wherein the treatment step is conducted under aerobic environment with stirring to convert the organic waste to an organic fertilizer.