The present invention relates to leader peptides, leader peptide fusion proteins, signal peptides, polynucleotides encoding the leader peptides and signal peptides, and to nucleic acid constructs, vectors and host cells comprising the polynucleotides as well as methods of producing a polypeptide of interest in host cells expressing the leader peptides in translational fusion with the polypeptide of interest.

The present invention relates to a method of preparing an anthocyanin oligomer using a coenzyme derived from an Aspergillus sp. strain, and more particularly to a method of preparing an anthocyanin oligomer by fermenting an anthocyanin monomer with a coenzyme of Aspergillus niger, which is a kind of Aspergillus sp. strain. According to the present invention, in order to overcome contamination problems during the culturing process using Aspergillus niger, a coenzyme of Aspergillus niger is extracted and the fermentation process is performed using the same, whereby an anthocyanin oligomer characterized by reduced concern of contamination and superior radical-scavenging effects, compared to existing anthocyanin monomers, can be produced. Also, an anthocyanin oligomer, obtained through fermentation using glucosidase as an enzyme contained in the coenzyme, can exhibit excellent fermentation efficiency and radical-scavenging ability, and polymerization of the anthocyanin oligomer can be confirmed even upon the fermentation of the enzyme including glucosidase.

A high temperature resistant mannanase mutant, comprising at least one mutation site among D49C/I/N, G58D/E/S, H90L, T99Y, K120Y, N124P, T130D/N/P/Q/R/S, Y132F, S136M, S140L/K, T148K, F206Y, A212N/S/P, L257F, S263R, D268A/E/G/P, G273P, S302M/E, S306M/Q, T315P, T353L/F, D360E/N/K, and A362R. The high temperature resistant mannanase mutant has heat resistance significantly higher than the wild type mannanase, and can be widely used in the field of feeds.

The present invention provides a method of preparing a furan derivative comprising the steps of (a) converting a monosaccharide to provide a keto-intermediate product; and (b) dehydrating the keto-intermediate product to provide a furan derivative; wherein the keto-intermediate product is pre-disposed to forming keto-furanose tautomers in solution. The method may further comprising a step of oxidizing the furan derivative to provide a furandicarboxylic acid or a furandicarboxylic acid derivative.

The present invention belongs to the technical field of biological fermented feeds, in particular to a two-step production method of a mycoprotein biological feed by fermenting corn husks. The present invention provides a preparation method for a mycoprotein biological feed, which comprises: inoculating Aspergillus niger microbial agent on a first fermentation substrate for first fermentation, to obtain a yeast seed; and inoculating a compound microbial agent in a second fermentation substrate to perform second fermentation. The compositions of the first fermentation substrate, the second fermentation substrate and the compound microbial agent are defined. The degradation of cellulose and hemicellulose in the corn husk raw material can be maximized and the protein content is increased through two fermentations, while taking into account the cost. A preparation method of the high-quality protein feed is provided for the feed industry.

The present application provides a glucose oxidase mutant, the glucose oxidase mutant has at least one of the following mutations compared to wild-type glucose oxidase: G108K, G419K; or the glucose oxidase mutant has at least one of the following mutations compared to wild-type glucose oxidase: G108D, G419D; the amino acid sequence of the wild-type glucose oxidase is set forth in SEQ ID NO:1.

The disclosure discloses a production method for low-toxin, low-viscosity and enhanced-quality corn steep liquor protein powder with a special flavor, belonging to the field of biological technologies. By inoculating a corresponding proportion of 10.sup.6 CFU/mL Aspergillus niger spore suspension into corn steep liquor and cultivating it at a certain rotation speed and temperature, the viscosity of the corn steep liquor effectively reduced, and the aflatoxins contamination is significantly reduced. The degradation rate of AFB1, AFB2, AFG1, AFG2 reach 52.14%, 80.21%, 86.31%, 80.22%. After fermentation, the protein content and total amino acid content of the corn steep liquor are increased after drying, and it can be dried into corn steep liquor powder. The disclosure provides a technical reference for the safe and efficient utilization of the corn steep liquor, greatly reduces the transportation cost of the corn steep liquor, extends the product storage period, and improves the convenience in use.

The present disclosure features a method of modulating production of a small halogenated organic compound with a peroxidase enzyme, and related compositions and methods of use thereof.

An active polysaccharide compound nutrient for boosting immunity and relieving fatigue, including 10-12 parts by weight of lentinan, 8-10 parts by weight of laminarin, 5-8 parts by weight of Lycium barbarum, 10-15 parts by weight of Mytilus edulis extract, 10-15 parts by weight of organic selenium protein powder, 8-10 parts by weight of walnut kernel, 3-5 parts by weight of black soybean powder, 2-3 parts by weight of Panax quinquefolius extract, 1-2 parts by weight of Schisandra chinensis, 1-2 parts by weight of Ophiopogon japonicus, 1-2 parts by weight of nutritional yeast, 5-10 parts by weight of L-arabinose, 5-8 parts by weight of xylitol and 0.2-0.3 part by weight of pancreatin. The active polysaccharide compound nutrient is prepared by subjecting raw materials to crushing, mixing, stirring, dissolving with deionized water, concentrating and drying under reduced pressure.

The present invention deals with the formation of Natural Organic Nano-Fertilizers with the chelated nano-nutrients to balance plant nutrition; improve water holding capacity, soil health improvement, sustainable productivity and quality improvement. The present invention involves production of eco-friendly and low cost process for the synthesis of nanoparticles of nano-nutrients by utilizing microorganisms comprising of two lab adapted strains of Aspergillus species, one lab adapted strain of Lactobacillus sp. and lactate, gluconate and proteinate salts as a source of nutrient leads to formation of metal nanoparticles (Zn, Mg, Fe and P) with the size of <20 nms. The present invention increases 12-20% of crop yield, stress tolerance of the crops, nutrient mobilization increases and 3 fold increase in nutrient use efficiency.