The present invention relates to the field of dihydromyricetin extraction and purification, and in particular to a dihydromyricetin extraction and purification process. Technical problem: the dihydromyricetin extraction and purification process aims to resolve the technical problems of increased cost of subsequent impurity removing and safety risks in high-temperature centrifugation in the prior art. Technical solution: a dihydromyricetin extraction and purification process: step 1: weighing a raw material, adding an extraction solvent in an amount 5 times that of the raw material to perform reflux extraction, concentrating the filtrate to an extract, and recovering acetone; step 2: resting for crystallization for 24 hours; step 3: performing suction filtration to obtain light-green sediment underneath, and dry the sediment; step 4: adding 5%-10% activated carbon for decolorization and impurity removing; step 5: performing suction filtration; step 6: drying to obtain white powder of dihydromyricetin; and step 7: detecting a content with HPLC.