Disclosed herein, interalia, are compounds, compositions, and methods of using the same for the sequencing of a nucleic acid.

The present invention relates to compounds comprising a salt of a diphosphate phosphoramidate of a nucleoside drug, e.g. clofarabine. The compounds are useful in the treatment of cancer, e.g. leukemia.

The use of described compounds or pharmaceutically acceptable salts or compositions thereof for the treatment of a host infected with an RNA virus other than HCV, or other disorder more fully described herein.

The use of described compounds or pharmaceutically acceptable salts or compositions thereof for the treatment of a host infected with an RNA virus other than HCV, or other disorder more fully described herein.

The invention is directed to a method for production of a deoxynucleotide triphosphate according to general formula (7)

##STR00001## wherein B is a nucleobase and R is an alkyl group having 1 to 4 carbon atoms and n is an integer from 1 to 10 by reaction of reaction precursor of compound 6

##STR00002## wherein R.sub.1, R.sub.2 and R.sub.3 are independently H, O-Alkyl with alkyl residues having 1 to 4 carbon atoms or halogen and n is an integer from 1 to 10 with dialkyl(alkylthio)sulfonium salt (8B)

##STR00003## wherein R.sub.4, R.sub.5 R.sub.6 are independently alkyl residues having 1 to 4 carbon atoms and X is selected from the group consisting of BF.sub.4.sup.−, F.sup.−, Cl.sup.−, Br.sup.−, I.sup.−, SO.sub.4.sup.2−, SO.sub.3.sup.2− in aqueous solution having a pH between 3 and 7.

The invention is directed to a method for production of a deoxynucleotide triphosphate according to general formula (7)

##STR00001## wherein B is a nucleobase and R is an alkyl group having 1 to 4 carbon atoms and n is an integer from 1 to 10 by reaction of reaction precursor of compound 6

##STR00002## wherein R.sub.1, R.sub.2 and R.sub.3 are independently H, O-Alkyl with alkyl residues having 1 to 4 carbon atoms or halogen and n is an integer from 1 to 10 with dialkyl(alkylthio)sulfonium salt (8B)

##STR00003## wherein R.sub.4, R.sub.5 R.sub.6 are independently alkyl residues having 1 to 4 carbon atoms and X is selected from the group consisting of BF.sub.4.sup.−, F.sup.−, Cl.sup.−, Br.sup.−, I.sup.−, SO.sub.4.sup.2−, SO.sub.3.sup.2− in aqueous solution having a pH between 3 and 7.

The invention provides a novel method for producing an oligonucleotide using a nucleoside or oligonucleotide that is easy to isolate and has high storage stability. The oligonucleotide production method includes a step of subjecting a nucleoside or oligonucleotide having a pseudo solid phase-protecting group in at least one location selected from the group consisting of 2′-position, 3′-position, 5′-position and a nucleobase moiety and having a 5′-hydroxyl group or a 3′-hydroxyl group, to H-phosphonation to convert the 5′-hydroxyl group or the 3′-hydroxyl group into an H-phosphonated form.

The invention provides a novel method for producing an oligonucleotide using a nucleoside or oligonucleotide that is easy to isolate and has high storage stability. The oligonucleotide production method includes a step of subjecting a nucleoside or oligonucleotide having a pseudo solid phase-protecting group in at least one location selected from the group consisting of 2′-position, 3′-position, 5′-position and a nucleobase moiety and having a 5′-hydroxyl group or a 3′-hydroxyl group, to H-phosphonation to convert the 5′-hydroxyl group or the 3′-hydroxyl group into an H-phosphonated form.

There is described herein, a method for inducing Tc22 lineage T cells from a population of CD8+ T cells, the method comprising: a) providing a population of CD8+ T cells; b) activating the population; and c) culturing or contacting the population of CD8+ T cells with Coenzyme A.

A labeled nucleotide includes a nucleotide, a linking molecule attached to a phosphate group of the nucleotide, and a redox-active charge tag attached to the linking molecule. The redox-active charge tag is to be oxidized or reduced by an electrically conductive channel when maintained in proximity of a sensing zone of the electrically conductive channel.