A system, including methods and compositions, for treatment of ischemia.

Polypeptide particles of the present invention are particles of a polypeptide derived from spider silk proteins, and have an average particle size of 1000 nm or less. A method for producing polypeptide particles of the present invention includes: a solution production step in which the polypeptide is dissolved in at least one solvent selected from the group consisting of DMSO, DMF, and these with an inorganic salt, so as to obtain a solution of the polypeptide; a step in which the solution produced in the solution production step is substituted with a water-soluble solvent so as to obtain an aqueous solution of the polypeptide; and a step in which the aqueous solution of the polypeptide is dried. Thereby, the present invention provides polypeptide particles suitable for application to a living body and capable of being applied to cosmetics, etc., while identifying the properties of the polypeptide particles, and a method for producing the same.

The present invention relates to a production method for a recombinant protein, the production method including a growth reduction step of reducing cell growth of recombinant cells which express a recombinant protein and a production step of producing the recombinant protein by culturing the recombinant cells in a protein production culture medium in a state of reducing the cell growth, in which the cell growth of the recombinant cells is reduced in the growth reduction step by using recombinant cells having at least one modified morphogenetic regulator as the recombinant cells.

Methods to prepare recombinant adeno-associated virus (AAV) capsids with altered tropism and compositions having AAVs with altered tropism are provided.

The present invention relates to C-terminal modified Protoxin II peptides that selectively inhibit the Nav1.7 sodium channel; the present invention also relates to pharmaceutical compositions useful for prophylactic or therapeutic treatment of a disorder responsive to the blockade of sodium ion channels, especially Nav1.7 sodium ion channels; the present invention further provides methods of treating a disorder responsive to the blockade of sodium channels, and particularly Nav1.7 sodium channels, in a mammal suffering from excess activity of said channels.

The present invention provides methods for producing a silk protein spinning dope solution suitable for producing high toughness fibres, the thus produced silk protein spinning dope solution, methods for producing fibres using said silk protein spinning dope solution.

Isolated polypeptides are disclosed comprising an amino acid sequence encoding a monomer of a fibrous polypeptide attached to a heterologous polysaccharide binding domain. Composites comprising same, methods of generating same and uses thereof are all disclosed.

Provided is a structural protein microbody that functions as a core for forming a protein nanofiber. There is provided a structural protein microbody including a structural protein, in which the structural protein microbody satisfies at least two of the following (i) to (iii): (i) a peak is present within a range of 480 to 500 nm in a fluorescence intensity measurement by thioflavin T staining; (ii) a peak is present in a region where Q is 0.15 or less in a modified Kratky plot of small angle X-ray scattering (SAXS); and (iii) the structural protein microbody is an aggregate of two or more structural protein molecules.

Provided is a water-absorbing and quick-drying property-imparting agent capable of easily imparting water-absorbing and quick-drying properties to various materials or articles in a simple process, and a method capable of easily imparting water-absorbing and quick-drying properties to predetermined materials or articles.

A water-absorbing and quick-drying property-imparting agent containing modified fibroin as an active ingredient, and a method for imparting water-absorbing and quick-drying properties to an article, the method including a step of incorporating modified fibroin into the article.

Disclosed are methods, compositions, and systems for transforming silkworms to produce spider silk and analogs of spider silk. In certain embodiments, the method may include inserting a DNA sequence coding for at least a portion of a spider silk fibroin polypeptide, or an analog of a spider silk fibroin polypeptide, positioned between at least a portion of the 5′ and 3′ ends of a silkworm fibroin gene to generate a fusion gene construct having a sequence that encodes for a polypeptide comprising both spider silk fibroin and silkworm silk fibroin sequences. In certain embodiments, the fused gene is able to replace a native gene present in the silkworm such that the transformed silkworm expresses a polypeptide comprising a spider silk fibroin polypeptide, or an analog thereof, and expresses significantly less of the native silkworm silk.