A method of generating offspring with a precharacterized genome is disclosed. In one embodiment, the method comprises the steps of (a) obtaining ungulate haploid embryonic cells, (b) deriving haploid embryonic outgrowth from the cells of step (a), (c) characterizing the genome of the haploid cells of step (b), and (d) deriving diploid embryos or offspring from the cells of step (c).

A computer-implemented method for predicting the likelihood an embryo will produce specific sex offspring by processing video image data derived from video of a target embryo. The method includes receiving image data derived from video of a target embryo taken at substantially real-time frame speed during an embryo observation period of time. The video contains recorded morphokinetic movement of the target embryo occurring during the embryo observation period of time. The movement is represented in the received image data and the received image data is processed using a model generated utilizing machine learning and correlated embryo outcome data to predict the likelihood the target embryo will produce specific sex offspring.

An artificial propagation method of Sinocyclocheilus rhinocerous, comprising placing female and male parent fish in a sterilized culture container in which a black cave simulant is placed in advance, strictly controlling the environmental parameters of the parent fish culture. The method realizes natural spawning and sperm production of S. rhinocerous under artificial conditions without using oxytocin. There is no oxytocin used in the artificial propagation, thus not only obtaining a higher fertilization rate and incubating rate, but also reducing the aquaculture cost. The operation is simpler and faster. At the same time, different feeds are fed in different growth stages according to the different growth characteristics of each stage of S. rhinocerous fry, thereby increasing the survival rate and reducing the deformity rate of fries. Therefore, the method provided by the present disclosure realizes efficient artificial propagation method of S. rhinocerous.

The present invention relates to improved assisted reproductive technology using highly purified menotropin (HP-hMG) to stimulate follicle development in controlled ovarian stimulation, particularly in women at risk of a high ovarian response to controlled ovarian stimulation.

Described herein is an egg yolk extract comprising freed/lysed yolk granules and/or yolk spheres. Also described is an egg yolk extract comprising the liquid contents of a shelled egg, wherein the liquid contents comprise yolk granules. Also described are an egg yolk extract comprising the liquid contents of yolk spheres; an egg yolk extract comprising the liquid contents of yolk granules; an egg yolk extract comprising ovalbumin and other anti-bactericidal proteins in egg white; and an egg yolk extract that supports cell proliferation at least equivalent to FBS when used at the same concentration in a cell culture medium. Related supplements, media, and methods are also described.

The method for automated cell positioning can include: sampling a video of a scene having a gamete, tracking the gamete, and positioning the gamete within a target region. The method can optionally include: determining attribute values for the gamete, selecting the gamete, reorienting the gamete, and/or manipulating the gamete, and/or any suitable steps.

Stabilized amorphous calcium carbonate (ACC) for treatment of several neurological, muscular and infertility diseases and conditions is provided. In particular, the stabilized ACC may be used in the treatment of axonal defects and muscular dystrophy. In addition, provided are improved methods used in assistant reproductive technology. Examples of such methods are in vitro fertilization and improvement of sperm quality. The improved IVF method, for example, comprises addition of the stabilized ACC to the cell culture medium in which the stages of fertilization and embryo development occurs.

Disclosed are methods and compositions for in situ germline genome engineering. The disclosed methods and compositions may be utilized for germline genome engineering in a subject having a reproductive organ containing a fertilized zygote, via: (i) isolating or obtaining the reproductive organ from the subject after a time period following insemination of the subject; (ii) introducing a reagent composition into the reproductive organ, the reagent composition comprising a nuclease system and/or an exogeneous polynucleotide; and (iii) electroporating the reproductive organ.

A novel piezo-driven cell injection system with force feedback overcomes the unsatisfied force interaction between the pipette needle and embryos in conventional position control. By integrating semiconductor strain-gage sensors for detecting the cell penetration force and the micropipette relative position in real time, the developed cell microinjection system features high operation speed, confident success rate, and high survival rate. The effectiveness of the developed cell injection system is experimentally verified by penetrating zebrafish embryos. The injection of 100 embryos are conducted with separate position control and force control. Results indicate that the force control enables a survival rate of 86%, which is higher than the survival rate of 82% produced by the position control in the same control environment. The experimental results quantitatively demonstrate the superiority of force control over conventional position control for the first time.

Provided is a method for efficiently proliferating an influenza virus serving as a material for vaccine in a host.

A method for proliferating an influenza virus in a host, comprising a step of inhibiting transfer of Bax in a host cell to the inner mitochondrial membrane.