A device for selecting sperm includes a stack of a plurality of layers of a material. The stack has an inlet end and an outlet end. Each layer of the material includes a plurality of sperm selection microchannels. Each sperm selection microchannel has a respective microchannel inlet at the inlet end of the stack and extends to a respective microchannel outlet at the outlet end of the stack.

The present invention relates to a composition comprising serotonin which is to be added into the wash solution for incubating the sperms during and after washing, intended for increasing the sperm velocity in insemination and in vitro fertilization methods to select healthy sperms and increase the success in fertilization, said invention discloses the composition increasing the sperm velocity, comprising 8 parts of serotonin, 10 000 parts of vitamin B9, 10 000 parts of vitamin B12, 10 000 parts of vitamin C, 10 000 parts of vitamin D, 10 000 parts of vitamin E, 10 000 parts of zinc mineral and 10 000 parts of selenium mineral by weight in an HTF based sperm wash medium containing Hepes buffer, and the preparation steps thereof.

Compositions and methods for improving embryo development, treating idiopathic male factor infertility, and enabling infertile/sub-fertile/sterile men to father their own genetic offspring are provided. Typically, the methods include administering into a male or female gamete or fertilized embryo an effective amount of a compound that increases bioavailability of a TET protein to improve development of an embryo resulting from fertilization of the female gamete by a male gamete. The compound can be administered into the gamete or embryo before, during, or after fertilization. The compound can be administered by an injection such as intracytoplasmic injection. The compound and the male gamete can be administered in combination by intracytoplasmic sperm injection. Methods of making male gametes, and methods of modifying the genome of a male gamete or embryo using an effective amount of a gene editing composition to correct a gene mutation or anomaly in the genome thereof are also provided.

A system and method for sorting sperm is provided. The system includes a housing and a microfluidic system supported by the housing. The system also includes an inlet providing access to the microfluidic system to deliver sperm to the microfluidic system and an outlet providing access to the microfluidic system to harvest sorted sperm from the microfluidic system. The microfluidic system provides a flow path for sperm from the inlet to the outlet and includes at least one channel extending from the inlet to the outlet to allow sperm delivered to the microfluidic system through the inlet to progress along the flow path toward the outlet. The microfluidic system also includes a filter including a first plurality of micropores arranged in the flow path between the inlet and the outlet to cause sperm traveling along the flow path to move against through the filter and gravity to reach the outlet.

A system and method for sorting sperm is provided. The system includes a housing and a microfluidic system supported by the housing. The system also includes an inlet providing access to the microfluidic system to deliver sperm to the microfluidic system and an outlet providing access to the microfluidic system to harvest sorted sperm from the microfluidic system. The microfluidic system provides a flow path for sperm from the inlet to the outlet and includes at least one channel extending from the inlet to the outlet to allow sperm delivered to the microfluidic system through the inlet to progress along the flow path toward the outlet. The microfluidic system also includes a filter including a first plurality of micropores arranged in the flow path between the inlet and the outlet to cause sperm traveling along the flow path to move against through the filter and gravity to reach the outlet.

The present application relates to a microfluidic system and its method for use for the separation of motile sperm from immotile sperm or motile bacteria from immotile bacteria. The system includes a housing having a first end, and a second end, with a passage connecting the first and second ends. There is an inlet at the first end of the housing for charging fluids into the passage and an outlet at the second end of said housing for discharging fluids from the passage. There are one or more corrals within the passage, each of the corrals including a closed side and a partially open side. The closed side of the corrals is closer to the first end than the partially open side, with the closed side and partially open side defining between them a confinement region suitable for retaining motile sperm or motile bacteria.

This disclosure provides improved method, kits, and systems and for isolating and enriching spermatogonial stem cells (SSCs) from livestock animal testicular tissue. In one aspect, the disclosure provides a method for enriching SSCs from a population of testis-derived cells containing at least one SSC, where the method comprises contacting the population of testis-derived cells to a culture media comprising, or that is preconditioned with, endothelial feeder cells, and maintaining culture conditions suitable for SSC cell maintenance and enrichment. In some embodiments, the media and culture conditions comprise one or more growth factors selected from GDNF, FGF2, SDF-1a, CSF-1, FDGF, NGF, and TGF-β, in any combination. In exemplary embodiments, the SSCs are porcine or bovine SSCs.

An embryo, gamete or stem cell culture medium comprising: a) acetyl-carnitine at a concentration of about 5 to about 50 μM; and b) lipoic acid or a derivative thereof at a concentration of about 2.5 to about 40 μM. The culture medium may optionally further comprises acetyl-cysteine at a concentration of about 5 to about 50 μM.

System and method includes a body having a central microchannel separated by one or more porous membranes. The membranes are configured to divide the central microchannel into a two or more parallel central microchannels, wherein one or more first fluids are applied through the first central microchannel and one or more second fluids are applied through the second or more central microchannels. The surfaces of each porous membrane can be coated with cell adhesive molecules to support the attachment of cells and promote their organization into tissues on the upper and lower surface of the membrane. The pores may be large enough to only permit exchange of gases and small chemicals, or to permit migration and transchannel passage of large proteins and whole living cells. Fluid pressure, flow and channel geometry also may be varied to apply a desired mechanical force to one or both tissue layers.

The present disclosure relates generally to potassium channel blocker compounds and compositions thereof; and methods for improving reproductive cell viability and quality, during and/or after one or more of staining, freezing, thawing, cell sample enrichment, packaging, or in vitro fertilization using the potassium channel blocker compounds.