The invention relates to a device (1) for preparing a cell suspension comprising an enzyme reservoir (10), a first purification compartment (20) for receiving a first purification material (P1), wherein the first purification compartment (20) is in fluid connection or can be brought into fluid connection with the enzyme reservoir (10), a syringe (40) comprising a barrel (41) defining a barrel compartment (45) and a piston (42) which is movably arranged in the barrel compartment (45), wherein the syringe (40) comprises a syringe inlet (43) connected to the barrel compartment (45), wherein the syringe inlet (43) is in fluid connection or can be brought into fluid connection with the first purification compartment (20), and wherein the syringe (40) comprises a syringe outlet (44), and wherein the device (1) comprises a flow path from the enzyme reservoir (10) via the first purification compartment (20) to the syringe inlet (43). Also provided herein are methods for preparing a cell suspension.

The present disclosure relates to a method of generating bioartificial reptile leather by in vitro culturing of isolated cells the chorioallantois of hatched reptile eggs. The disclosure allows production of reptile leather without the ethical issues of conventional reptile farming and the trapping and killing of reptiles for their skin. Furthermore the disclosure allows production of reptile leather from species that are not abundantly available as skin products, such as from endangered species.

Provided herein are methods and compositions for enhancing an immune response and/or for the treatment of an immune-related condition in an individual, e.g., cancer, comprising killing, disabling, or depleting non-stimulatory myeloid cells using an antigen binding protein such as an antibody or antigen binding fragment thereof.

A preparation method of a human scalp hair follicle single cell suspension includes the following steps: step 1, a fresh isolated scalp tissue is pretreated with a digestive enzyme solution in a low temperature environment; step 2, the hair follicles can be effectively dissociated by digestion with the digestive enzyme solution at 30-37 C.; step 3, the dissociated hair follicles are digested with pancreatin-EDTA to obtain a human scalp hair follicles single cell suspension. In addition, the invention discloses a human scalp hair follicle single cell suspension prepared by the above method and use thereof. The method of the present invention is short in time and the hair follicles dissociated are high in integrity, and the subsequent isolated single cell populations are large in number and high in vitality, which solves the problems of incomplete separation of hair follicles from isolated scalp tissue, long cell extraction time, and low cell viability.

As a technique for proliferating neural crest cells without reducing differentiation capacity, provided is a method for producing neural crest cells, comprising the steps of: (1) obtaining neural crest cells; and (2) suspension-culturing the neural crest cells in a medium comprising a GSK3? inhibitor and a basic fibroblast growth factor (bFGF), wherein the medium comprises the GSK3? inhibitor with a concentration that exhibits an effect equivalent to that exhibited by CHIR99021 with a concentration of higher than 1 ?M and lower than 5 ?M.

Provided herein are methods and compositions for enhancing an immune response and/or for the treatment of an immune-related condition in an individual, e.g., cancer, comprising killing, disabling, or depleting non-stimulatory myeloid cells using an antigen binding protein such as an antibody or antigen binding fragment thereof.

Disclosed are compositions comprising cellular constructs comprising mesenchymal cells and epithelial cells. Also disclosed are methods of making the cellular constructs, methods of hair restoration, and kits. The invention also discloses parallel bio-printing systems and methods for making cellular constructs, such as cellular constructs comprising mesenchymal cells and epithelial cells.

Forced expression of a handful of transcription factors (TFs) can induce conversions between cell identities; however, the extent to which TFs can alter cell identity has not been systematically assessed. Here, we assembled a human TFome, a comprehensive expression library of 1,578 human TF clones with full coverage of the major TF families. By systematically screening the human TFome, we identified 77 individual TFs that induce loss of human-induced-pluripotent-stem-cell (hiPSC) identity, suggesting a pervasive ability for TFs to alter cell identity. Using large-scale computational cell type classification trained on thousands of tissue expression profiles, we identified cell types generated by these TFs with high efficiency and speed, without additional selections or mechanical perturbations. TF expression in adult human tissues only correlated with some of the cell lineage generated, suggesting more complexity than observation studies can explain.

The present invention relates to novel melanocytes and melanoblasts. In addition, the present invention relates to a novel method for producing melanocytes and melanoblasts. Specifically, provided is novel melanocytes of which gene expression, melanin content, and tyrosinase activity are different from those of conventional melanocytes. Even more specifically, provided is novel melanoblasts of which the gene expression, the melanin content the tyrosinase activity, and the protein expression are different from those of conventional melanocytes. Additionally, provided is a novel method for producing melanocytes or melanoblasts by culturing keratinocytes.

Forced expression of a handful of transcription factors (TFs) can induce conversions between cell identities; however, the extent to which TFs can alter cell identity has not been systematically assessed. Here, we assembled a human TFome, a comprehensive expression library of 1,578 human TF clones with full coverage of the major TF families. By systematically screening the human TFome, we identified 77 individual TFs that induce loss of human-induced-pluripotent-stem-cell (hiPSC) identity, suggesting a pervasive ability for TFs to alter cell identity. Using large-scale computational cell type classification trained on thousands of tissue expression profiles, we identified cell types generated by these TFs with high efficiency and speed, without additional selections or mechanical perturbations. TF expression in adult human tissues only correlated with some of the cell lineage generated, suggesting more complexity than observation studies can explain.