The disclosure relates to methods of preparation of fetal nucleated red blood cells (NRBCs) from biological samples for diagnostic testing.

Disclosed herein, are recombinant polypeptides comprising one or more homologous amino acid repeats; and, non-immunogenic bioconjugates comprising recombinant polypeptides comprising one or more homologous amino acid repeats and one or more therapeutic agents. Also, disclosed herein are pharmaceutical compositions including the recombinant polypeptides; and methods of administering the recombinant polypeptides to patients for the treatment of cancer or infections.

A storage solution for packed red blood cells is disclosed where the solution is alkaline and has a viscosity at 4° C. greater than about 20 millipascal seconds. In one embodiment, the storage solution comprises from about 0.1 percent to about 10 percent of hydroxy-propyl-methyl-cellulose. In another embodiment, the storage solution has a pH greater than about 8.

The disclosure relates to methods of preparation of fetal nucleated red blood cells (NRBCs) from biological samples for diagnostic testing.

Embodiments of the present invention relate to methods of preparing a cell, tissue, organ or plant for cryopreservation, wherein the method includes contacting the cell, tissue, organ or plant with a composition including sucrose and/or sucralose.

The present disclosure provides a method for producing erythroid cells and/or erythrocytes comprising culturing hematopoietic stem cells (HSCs) or erythroid cells with a population of immortalized mesenchymal stem cells (MSCs) or conditioned medium obtained from the immortalized MSCs, wherein the immortalized MSCs are genetically engineered with a survival gene. Also provided is a method of making a blood product for use in transfusions and a method for increasing hemoglobin synthesis.

Blood and platelet storage and rejuvenating compositions that include triciribine, triciribine metabolites, triciribine analogs, and mixtures of the same are disclosed. Such compositions can be useful in methods for treating (e.g., storing and rejuvenating) red blood cells and platelets.

The present invention relates to a method for in vitro production of red blood cells. The method for in vitro production of red blood cells, according to the present invention, specifies, by cell size, the maturation step of cells exhibiting optimal effects in an agitation-type culture, so that even if an expert does not always identify cell shape, automated processes of culturing are possible, and thus automation in bioreactors is possible in the mass-production of uniform quality and red blood cells.

The invention includes compositions and methods related to multimodal therapies, e.g., for treating a cancer. A multimodal therapy described herein provides and/or administers a plurality of agents that function in a coordinated manner to provide a therapeutic benefit to a subject in need thereof, e.g., a subject having a cancer.

A method of controlling red blood cell production includes contacting red blood cell precursors with a composition including a zinc chelator, a composition including an inhibitor of a zinc importer protein, or a combination thereof; wherein the contacting inhibits survival of the red blood cell precursors, inhibits terminal differentiation of the red blood cell precursors to mature red blood cells, or a combination thereof.