Described herein are decellularized extracellular matrix for mechanically supporting engineered vascular grafts. Methods are provided for fabricating all-natural, non-immunogenic, strong products that do not rely on common plastic supports. Also provided are bench-top models of atherosclerosis. Embodiments provide completely inclusive models that contain all steps of atherosclerosis, including late-stage disease processes. Example models utilize tissue engineered blood vessels (TEBV); stages of atherosclerosis are induced for instance by application of oxidized low-density lipoprotein (oxLDLs) (early-stage), followed by macrophage introduction (early-stage), and induction of calcification using calcified protein particles (CPPs; late-stage). Also provided are kits useful to investigate disease processes and better patient treatment options, including new drug development.

A method for producing a three-dimensional cell structure having a vascular network, including preparing a mixture of a cationic substance, a polyelectrolyte, an extracellular matrix component, and a cell population including endothelial cells, collecting, from the mixture, a cell aggregate including the cell population, the cationic substance, the polyelectrolyte, and the extracellular matrix component, and culturing a collected cell aggregate in a medium. The mixture includes the extracellular matrix at a concentration of 1.0×10.sup.−8 mg/mL or more and less than 2.5×10.sup.−2 mg/mL.

A network (100) for replacement of a living tissue, said network is a scaffold-free artificial hollow biological tissue network comprising a plurality of longitudinal multicellular aggregates (11) arranged in a plurality of bioprinted layers (22) which are located on top of one another, further comprising an inner surface (20) and an outer surface (21), wherein at least one of said bioprinted layers (22) is in shape of a planar closed loop such that a conduit for conveying fluids is defined, and said longitudinal multicellular aggregate (11) is a mixture of at least two cell types. Also a method for obtaining said longitudinal multicellular aggregate, and a further method for biomodeling and planning said network are proposed.

Provided herein is a drug delivery device and composition, such as a particle, comprising conditioned medium. Also provided herein is a method of preparing polymeric particles for release of conditioned medium. Further, a tissue growth scaffold comprising particles for release of conditioned medium is provided.

Vascularizing cell aggregates or tissue segments in a microfluidic device by filling a chamber within the device with a matrix that allows for endothelial sprouting; creating at least three voids within the matrix, of which at least two outer voids are lumenally connected to separate perfusion paths within the device and at least one additional void is positioned in between the at least two outer voids; endothelializing the at least two outer voids; introducing at least one cell type, matrix material, tissue segment, or combinations thereof into the void between the two outer voids; and using vascular growth factors to induce the endothelial cells to sprout into the matrix until the at least three voids are interconnected by endothelial sprouts.

The present invention is directed to indirect ultrasonic cavitation-derived perivascular cells, to methods of use of a perivascular cell composition, to a method of processing a tissue and to an apparatus for the processing of a tissue. The methods include the mechanic indirect ultrasonication of a cellular non-structural tissue, and produce a perivascular fraction which includes perivascular cells. The methods of use are directed to the treatment of a variety of diseases and disorders and to the improvement of a tissue in a subject. The apparatus is provided for the processing of cellular non-structural tissue.

The present invention relates to endothelial cells and methods of generating endothelial cells from pluripotent stem cells.

The present disclosure relates to a simple, fast, and low cost method for 3D microvascular imaging, termed “scatter labeled imaging of microvasculature in excised tissue” (SLIME). The method can include perfusing a contrast agent through vasculature of a tissue sample. The contrast agent can include colloids and a dispersant. After the contrast agent is perfused through the vasculature, the vasculature of the tissue sample can be treated with a molecule that cross links with at least a portion of the dispersant to form a sticky, non-Newtonian polymer that prevents leakage of the contrast agent out of the vasculature of the tissue sample. The tissue sample can then be immersed in a solution comprising a clearing agent and subsequently imaged.

Provided are methods and compositions for tissue engineering including methods and compositions for the generation of vascularized organoids in vitro.

Methods and compositions are provided for regenerating smooth muscle tissue by the provision of a purified population of epicardial-derived pericytes, where the smooth muscle tissue may comprise, without limitation, coronary artery tissue; kidney tissue, etc. Compositions and kits for practicing the methods and/or for use with the systems of the disclosure are also provided.