Provided herein is an in vitro model of the blood brain barrier. In some embodiments, the model includes: an endothelial cell layer, and brain tissue layer comprising neuronal cells, and optionally one or more of astrocytes, pericytes, oligodendrocytes, and microglia. In some embodiments, the model further comprises a porous membrane between said endothelial cell layer and the neuronal cell layer. A microfluidic device comprising the same and methods of use thereof are also provided.

The present disclosure is related to a perfusable-type bio-dual proximal tubule cell construct and a producing method thereof capable of applying an in vitro artificial organ model configured to include a first bioink comprising a decellularized substance derived from a mammalian kidney tissue and human umbilical vascular endothelial cells (HUVECs) and a second bioink comprising the decellularized substance and renal proximal tubular epithelial cells (RPTECs), wherein the first bioink and the second bioink are coaxial and printed in tubular constructs having different inner diameters.

According to the present disclosure, it is possible to use the renal proximal tubule-on-a-chip as a bioreactor capable of observing a biological drug reaction similar to a real drug by perfusing various drugs to the renal proximal tubule-on-a-chip.

Devices, systems, and techniques are described for printing pre-aligned microtissues into larger tissue constructs. For example, a method of printing a tissue construct includes aligning cells in a first direction to create pre-aligned microtissues, suspending the pre-aligned microtissues in a liquid to create a bioink, and depositing the pre-aligned microtissues in a second direction to create the tissue construct.

Embodiments of the present specification provide systems and methods for holding one or more tissues, such as veins, such that the tissue remains open in a chamber while undergoing orbital shaking with various solutions and to allow uniform treatment during a decellularization process. A frame is held on a stand to which the tissues are attached and comprises a tension inducing mechanism to cause the tissues to controllably stretch. The frame is removed from the stand, with the tissue attached to it, and placed in a decellularization chamber for uniform treatment of the tissue.

The present subject matter relates to techniques for mimicking the hemostasis microenvironment and predicting the effects of drugs on hemostasis. The disclosed system can include a top layer including a plurality of top rails, and a bottom layer including a plurality of bottom rails, wherein the top layer and the bottom layer are configured to be coupled, wherein the plurality of top rails and bottom rails are configured to form a plurality of channels comprising an intravascular channel configured to circulate a first solution, an extravascular channel configured to circulate a second solution, and a vessel wall channel including a tissue factor in a hydrogel.

The present invention relates to a method for producing a three-dimensional tissue construct, the method comprising: a culture step of culturing cells in a culture liquid comprising fragmented extracellular matrix components, fibrin, and an aqueous medium.

Provided herein is a method for assessing angiogenic effects of a test composition, the method including: providing human microvessel (MV) fragments selected to correspond to a desired patient profile; embedding the human MV fragments in a gel matrix of a three dimensional (3D) in vitro culture; providing serum free media to the 3D in vitro culture; contacting the 3D in vitro culture comprising embedded human MV fragments with a test composition; and assessing the angiogenic effects of the test composition by measuring at least one angiogenic growth parameter of the 3D in vitro culture comprising embedded human MV fragments. Also provided herein are 3D in vitro cultures useful in the disclosed methods.

Disclosed herein are recellularized livers prepared from decellularized liver extracellular matrices. Also disclosed herein are kits and systems comprising a recellularized liver as described herein. Also disclosed herein are methods of recellularizing livers from decellularized liver extracellular matrices.

A modular synthetic tissue-graft scaffold (10) includes one or more nominally identical scaffold cages (12) configured to facilitate regrowth of tissue of an organism in and around the scaffold cages. Each scaffold cage comprises a volumetric enclosure (18) bounded by a perforated wall structure (40). A recess (24) formed at one end of the volumetric enclosure defines an inner stepped coupling surface. An annular raised portion (26) positioned at the other end of the volumetric enclosure forms an outwardly projecting stepped seating surface sized to form a complementary matable surface to the inner stepped coupling surface for whenever an inner stepped coupling surface of another one of the cages is placed on the outer stepped seating surface of the scaffold cage. Corridors (46) extending through the perforated wall structure and communicating with passageways (54) within the volumetric enclosure enable migration of material within and out of the scaffold cage.

The disclosure provides 3D bioprinted test beds and methods of making the 3D bioprinted teste beds, methods of using the 3D bioprinted test beds for testing and/or comparatively testing two or more test compounds on cell growth and/or behavior, as well as biocompatible methacrylated hyaluronic acid-based bioinks for printing the 3D test beds and/or other articles. The 3D test beds and bioinks include a hydrogel material/precursor and can include extracellular matrix components.