An isolated and/or purified -glucanotransferase from Exiguobacterium Acetylicum, recombinantly engineered variants thereof, active fragments thereof, synthetic nucleic acids encoding the -glucanotransferase and variants thereof, host cells comprising the synthetic nucleic acids, and compositions comprising the -glucanotransferase are provided. Methods of using the compositions include the manufacture of oligosaccharides.

A process for enabling the production of a particulate composition containing crystalline trehalose dihydrate is provided. Including allowing an -glycosyltrehalose-forming enzyme to act on liquefied starch derived from a microorganism of the genus Arthrobacter and a trehalose-releasing enzyme derived from a microorganism of the genus Arthrobacter along with a starch debranching enzyme and a cyclomaltodextrin glucanotransferase; allowing glucoamylase to act on the resulting mixture to obtain a saccharide solution containing ,-trehalose; precipitating crystalline ,-trehalose dihydrate from the above saccharide solution; collecting the precipitated crystalline ,-trehalose dihydrate by a centrifuge; and ageing and drying the collected crystals. Cyclomaltodextrin glucanotransferase derived from a microorganism of the genus Paenibacillus or a mutant enzyme thereof is used to increase the ,-trehalose content in the saccharide solution to over 86.0% by weight, on a dry solid basis, without passing through a fractionation step by column chromatography.

A process for enabling the production of a particulate composition containing crystalline trehalose dihydrate is provided. Including allowing an -glycosyltrehalose-forming enzyme to act on liquefied starch derived from a microorganism of the genus Arthrobacter and a trehalose-releasing enzyme derived from a microorganism of the genus Arthrobacter along with a starch debranching enzyme and a cyclomaltodextrin glucanotransferase; allowing glucoamylase to act on the resulting mixture to obtain a saccharide solution containing ,-trehalose; precipitating crystalline ,-trehalose dihydrate from the above saccharide solution; collecting the precipitated crystalline ,-trehalose dihydrate by a centrifuge; and ageing and drying the collected crystals. Cyclomaltodextrin glucanotransferase derived from a microorganism of the genus Paenibacillus or a mutant enzyme thereof is used to increase the ,-trehalose content in the saccharide solution to over 86.0% by weight, on a dry solid basis, without passing through a fractionation step by column chromatography.

The present invention relates to high intensity sweetener glycosides which have been modified using a glycosyltransferase so as to reduce off-flavours. The invention also relates to uses of the modified high intensity sweetener glycosides and methods of production thereof.

A process for enabling the production of a particulate composition containing crystalline trehalose dihydrate is provided. Including allowing an -glycosyltrehalose-forming enzyme to act on liquefied starch derived from a microorganism of the genus Arthrobacter and a trehalose-releasing enzyme derived from a microorganism of the genus Arthrobacter along with a starch debranching enzyme and a cyclomaltodextrin glucanotransferase; allowing glucoamylase to act on the resulting mixture to obtain a saccharide solution containing ,-trehalose; precipitating crystalline ,-trehalose dihydrate from the above saccharide solution; collecting the precipitated crystalline ,-trehalose dihydrate by a centrifuge; and ageing and drying the collected crystals. Cyclomaltodextrin glucanotransferase derived from a microorganism of the genus Paenibacillus or a mutant enzyme thereof is used to increase the ,-trehalose content in the saccharide solution to over 86.0% by weight, on a dry solid basis, without passing through a fractionation step by column chromatography.

The present invention relates to a novel cyclodextrin glucanotransferase (CGTase) enzyme which is obtainable from Clostridium saccharoperbutylacetonicum N1-4, N1-4(HMT) or N1-504. The invention further relates to nucleic acids encoding the enzyme, vectors and host cells, and uses of the CGTase.

A process for enabling the production of a particulate composition containing crystalline trehalose dihydrate is provided. Including allowing an -glycosyltrehalose-forming enzyme to act on liquefied starch derived from a microorganism of the genus Arthrobacter and a trehalose-releasing enzyme derived from a microorganism of the genus Arthrobacter along with a starch debranching enzyme and a cyclomaltodextrin glucanotransferase; allowing glucoamylase to act on the resulting mixture to obtain a saccharide solution containing ,-trehalose; precipitating crystalline ,-trehalose dihydrate from the above saccharide solution; collecting the precipitated crystalline ,-trehalose dihydrate by a centrifuge; and ageing and drying the collected crystals. Cyclomaltodextrin glucanotransferase derived from a microorganism of the genus Paenibacillus or a mutant enzyme thereof is used to increase the ,-trehalose content in the saccharide solution to over 86.0% by weight, on a dry solid basis, without passing through a fractionation step by column chromatography.

The present invention relates to a novel cyclodextrin glucanotransferase (CGTase) enzyme which is obtainable from Clostridium saccharoperbutylacetonicum N1-4, N1-4(HMT) or N1-504. The invention further relates to nucleic acids encoding the enzyme, vectors and host cells, and uses of the CGTase.

A process for enabling the production of a particulate composition containing crystalline trehalose dihydrate is provided. Including allowing an -glycosyltrehalose-forming enzyme to act on liquefied starch derived from a microorganism of the genus Arthrobacter and a trehalose-releasing enzyme derived from a microorganism of the genus Arthrobacter along with a starch debranching enzyme and a cyclomaltodextrin glucanotransferase; allowing glucoamylase to act on the resulting mixture to obtain a saccharide solution containing ,-trehalose; precipitating crystalline ,-trehalose dihydrate from the above saccharide solution; collecting the precipitated crystalline ,-trehalose dihydrate by a centrifuge; and ageing and drying the collected crystals. Cyclomaltodextrin glucanotransferase derived from a microorganism of the genus Paenibacillus or a mutant enzyme thereof is used to increase the ,-trehalose content in the saccharide solution to over 86.0% by weight, on a dry solid basis, without passing through a fractionation step by column chromatography.

The present invention relates to a novel cyclodextrin glucanotransferase (CGTase) enzyme which is obtainable from Clostridium saccharoperbutylacetonicum N1-4, N1-4(HMT) or N1-504. The invention further relates to nucleic acids encoding the enzyme, vectors and host cells, and uses of the CGTase.