The present invention provides compositions of recombinant human lysosomal acid lipase having particular glycosylation patterns for internalization into target cells, a vector containing the nucleic acid encoding human lysosomal acid lipase, a host cell transformed with the vector, pharmaceutical compositions comprising the recombinant human lysosomal acid lipase and method of treating conditions associated with lysosomal acid lipase deficiency.

The present invention relates to a Lipase enzyme comprising an amino acid sequence that has at least 90% sequence identity with SEQ ID NO 1, or comprising a functional fragment thereof encompassing position Q55, with the proviso that the lipase enzyme has a substitution of an amino acid with a basic side chain at neutral pH in position Q55.

The present invention relates to a Lipase enzyme comprising an amino acid sequence that has at least 90% sequence identity with SEQ ID NO 1, or comprising a functional fragment thereof encompassing position Q55, with the proviso that the lipase enzyme has a substitution of an amino acid with a basic side chain at neutral pH in position Q55.

A process of stabilizing the activity of an enzyme against inactivation by water weathering are provided including associating one or more polymeric moieties of a polyoxyethylene having a molecular weight of 10,000 Daltons or greater with an enzyme to form a chemically modified enzyme; and dispersing said chemically modified enzyme in a base to form a water-stabilized active coating material.

A process of stabilizing the activity of an enzyme against inactivation by water weathering are provided including associating one or more polymeric moieties of a polyoxyethylene having a molecular weight of 10,000 Daltons or greater with an enzyme to form a chemically modified enzyme; and dispersing said chemically modified enzyme in a base to form a water-stabilized active coating material.

An object of the present invention is to provide an enzyme showing random transesterification ability, which is suitable for food applications. Provided is a random transesterification lipase having high heat resistance in oils and fats.

An object of the present invention is to provide an enzyme showing random transesterification ability, which is suitable for food applications. Provided is a random transesterification lipase having high heat resistance in oils and fats.

Described herein are peptides from secretory phospholipase A.sub.2-IB and antibodies that can be used to reduce the contribution of the gastrointestinal tract to inflammatory processes including systemic inflammatory responses. Specifically, antibodies that bind specifically a peptide from secretory phospholipase A.sub.2-IB prevent death in a mouse model of LPS-induced endotoxemia. The antibodies described herein are particularly useful to treat systemic inflammatory response syndromes, including sepsis.

Described herein are peptides from secretory phospholipase A.sub.2-IB and antibodies that can be used to reduce the contribution of the gastrointestinal tract to inflammatory processes including systemic inflammatory responses. Specifically, antibodies that bind specifically a peptide from secretory phospholipase A.sub.2-IB prevent death in a mouse model of LPS-induced endotoxemia. The antibodies described herein are particularly useful to treat systemic inflammatory response syndromes, including sepsis.

Described herein are anti-microbial and UV-protective biological devices and extracts produced therefrom. The biological devices include microbial cells transformed with a DNA construct containing genes for producing proteins such as, for example, zinc-related protein/oxidase, silicatein, silaffin, and alcohol dehydrogenase. In some instances, the biological devices also include a gene for lipase. Methods for producing and using the devices are also described herein. Finally, compositions and methods for using the devices and extracts to kill microbial species or prevent microbial growth and to reduce or prevent UV-induced damage or exposure to materials, items, plants, and human and animal subjects are described herein. Also disclosed are biological devices producing polyactive carbohydrates and carbo sugars, as well as compositions and articles incorporating both extracts from these devices and the anti-microbial and UV-protective extracts.