An engineered bacterium based on Bacillus subtilis is provided, a genome of the engineered bacterium includes a multi-enzyme element, and the multi-enzyme element comprises a class I -agarase element (E1 element), a class II -agarase element (E2 element), and an -neoagaro-diohydrolase element (E3 element). The engineered bacterium integrates agarase genes into the Bacillus subtilis genome, achieving cascade catalysis of agarose to produce active oligosaccharides and monosaccharides, thereby utilizing the biological activity of these active oligosaccharides and monosaccharides to treat intestinal inflammation. An engineered biofilm based on Bacillus subtilis is also provided, which includes the engineered bacterium and its secretions. Furthermore, a living material for multi-enzyme display and treatment of intestinal inflammation in mice is also provided, which includes the engineered bacterium or the engineered biofilm.

Sterile filtered liquid lactase composition comprising a lactase, preferably a neutral or acidic lactase, an anion selected from malate, tartrate, citrate, gluconate and/or EDTA and a cation selected from sodium and potassium. Preferably, the composition further comprises a polyol. Addition of the said anions to lactase improves filterability by reducing pressure in a filtration system. Preferably the filtration forms part of an in-line filtration system in the production of a dairy product.

The present invention relates to a method of producing neoagarotetraose (NA4) and neoagarohexaose (NA6) with high purity in large amounts by liquefaction of the substrate agarose using a thermostable GH16B -agarase derived from a novel agarolytic bacterium Cellvibrio sp. KY-GH-1 deposited under accession number KCTC 13629BP. The method of producing neoagarotetraose (NA4) and neoagarohexaose (NA6) with high purity in large amounts by liquefaction of the substrate agarose using a thermostable GH16B -agarase derived from a novel agarolytic bacterium Cellvibrio sp. KY-GH-1 deposited under accession number KCTC 13629BP according to the present invention may effectively produce NA4 and NA6 with high purity in large amounts by efficiently performing liquefaction of the substrate agarose, because the thermostable GH16B -agarase exhibits excellent enzyme activity at a high temperature equal to or higher than the agarose gelling temperature and has long-term thermal stability even at high temperatures.

The present application relates to sialylated glycoprotein compositions and methods of their use in treating various conditions and disorders.