The present invention relates to protein sequencing, more particularly the invention discloses improved aminopeptidases for single molecule protein sequencing and/or amino acid identification. Said aminopeptidases can enzymatically cleave off N-terminal amino acids and are highly suitable in a kinetics-based peptide sequencing approach. Based on the kinetics of the cleaving reaction or of the engagement between said aminopeptidases and peptide to be sequenced, information on the identity of the cleaved amino acids is provided.

The present invention relates to protein sequencing, more particularly the invention discloses improved aminopeptidases for single molecule protein sequencing and/or amino acid identification. Said aminopeptidases can enzymatically cleave off N-terminal amino acids and are highly suitable in a kinetics-based peptide sequencing approach. Based on the kinetics of the cleaving reaction or of the engagement between said aminopeptidases and peptide to be sequenced, information on the identity of the cleaved amino acids is provided.

Disclosed herein are systems and methods for manufacturing botulinum neurotoxin serotype E (BoNT/E) with improved yield and purity of BoNT/E. Disclosed herein are systems and methods for manufacturing BoNT/E drug substance.

Herein is reported a method for producing a bispecific antibody comprising the step of incubating (i) an antibody Fab fragment or a scFv antibody comprising within the 20 C-terminal amino acid residues the amino acid sequence LPX1TG (SEQ ID NO: 01), (ii) a one-armed antibody comprising a full length antibody heavy chain, a full length antibody light chain, and an Fc-heavy chain, whereby the full length antibody heavy chain and the full length antibody light chain are cognate antibody chains that thereof forms an antigen binding site, whereby the full length antibody heavy chain and the Fc-heavy chain are covalently linked to each other via one or more disulfide bonds forming an antibody hinge region, and whereby the Fc-heavy chain has an oligoglycine amino acid sequence at its N-terminus, and (iii) a Sortase A enzyme.

The present invention relates to a novel endoprotease, mutants thereof having binding but lacking or having reduced hydrolyzing activity, and use in methods of studying and isolating O-linked glycoproteins.

The present invention relates to a novel endoprotease, mutants thereof having binding but lacking or having reduced hydrolyzing activity, and use in methods of studying and isolating O-linked glycoproteins.

The present invention relates to novel proteases, more particularly to protease variants having improved activity compared to the protease of SEQ ID NO: 1 and the uses thereof for degrading polyester containing material, such as plastic products. The proteases of the invention are particularly suited to degrade polylactic acid, and material containing polylactic acid.

The present invention relates to novel proteases, more particularly to protease variants having improved activity compared to the protease of SEQ ID NO: 1 and the uses thereof for degrading polyester containing material, such as plastic products. The proteases of the invention are particularly suited to degrade polylactic acid, and material containing polylactic acid.

Provided is a genome editing system and method for gene editing at least one target sequence in a cell genome. The genome editing system comprises: (1) an expression construct comprising a Cas12f nuclease; and (2) an expression DNA sequence comprising a guide RNA corresponding to the Cas12f nuclease, and an expression construct of the targeting sequence of the target sequence. The gene editing system or method can accurately knock out a target gene from within a cell; in addition, in an in vitro cutting experiment, the target gene can be accurately cut.

The present invention pertains to: a botulinum toxin, epithelial cell growth factor, or hexapeptide fusion protein bound to skin tissues and cell-permeable peptides, or an epithelial cell growth factor mixed with skin tissues and cell-permeable peptides; and a composition comprising same. The fusion protein or the epithelial cell growth factor mixed with cell-permeable peptides has increased cell permeability compared to protein by itself, and is thus useful for improving the condition of skin, treating wrinkles, relieving muscle tension, and treating wounds.