Compositions, systems and methods are provided for conferring disease resistance to plant pathogens that use proteases to target plant substrate proteins inside plant cells. Briefly, the compositions, systems and methods are based upon plant substrate proteins that are targeted by pathogen-specific proteases and that activate nucleotide binding site-leucine rich repeat (NB-LRR) disease resistance proteins when cleaved by the protease. These substrate proteins are modified such that the endogenous protease recognition sequence is replaced by a protease recognition sequence specific to a different pathogen protease (i.e., a heterologous protease recognition sequence). The modified plant substrate protein therefore can be used in connection with its corresponding NB-LRR protein to activate resistance in response to cleavage by the heterologous pathogen-specific protease. When activated by the plant pathogen-specific protease, the pair initiates host defense responses thereto, including programmed cell death.

[Issues] To provide a method to produce diesel fuel that meets the standards of almost all countries in a short time.

[Solution] A method for producing diesel fuel having improved properties by mixing an aqueous enzyme solution containing 0.01 to 0.1% by weight of a lipase enzyme as a first enzyme with a water-soluble alcohol so as to be an aqueous alcohol solution having an alcohol concentration of from 20 to 35%, comprises adding a second enzyme solution in light oil having 0.001 to 0.01% by weight of pineapple-derived enzyme containing bromelain, dissolved in light oil to said base oil.

The present disclosure relates generally to generation of a recombinant enzyme with cyclization activity and its use for generating cyclic peptides as well as linear peptide conjugates.

The present invention provides a method for increasing nitrogen-use efficiency and/or nitrogen-utilisation efficiency in a plant comprising modifying the plant by increasing the activity or expression of an ethylene-dependent gravitropism-deficient and yellow green protein (EGY) in said plant.

Provided in the present disclosure are a milk coagulant, a method for obtaining asclepain of Asclepias Linn. and cysteine protease B of Calotropis R. Br., as well as a method of producing cheese. The milk coagulant includes at least one of the asclepain of Asclepias Linn. and the cysteine protease B of Calotropis R. Br.

The present invention addresses the problem of indicating active-ingredient classes which can treat virus diseases caused by coronaviruses in a human or animal. Said treatment includes the acute treatment of an already existing virus disease and the prophylaxis of same. The present invention relates to a bromelain protease, bromelain, jacalin-like lectin, extract from the stem and/or the fruit of a pineapple plant, combination preparation, bromelain protease inhibitor, protein/protease mix, and glycated bromelain protein formed by exogenous non-enzymatic glycation, for use in the treatment or prophylaxis of virus infections caused by coronaviruses in a human or animal.

Methods for wound debridement and specifically methods of debridement of chronic wounds. These methods provide topically applying to a wound site a debriding formulation in the form of a hydrogel that includes a proteolytic enzyme mixture obtained from bromelain and a water-soluble gelling agent, with the debriding formulation being applied to the wound site up to ten times over a period of up to four weeks, thereby achieving debridement of chronic wounds.

The present invention relates to novel compositions comprising proteolytic enzymes and fining agents as well as methods for the treatment and/or prevention of cancer using these compositions.

Provided herein are compositions, foods comprising nepenthesin or a derivative thereof and methods of using nepenthesin or a derivative thereof for modulating gluten intolerance and related conditions, such as celiac disease. Further provided herein are pharmaceutical compositions comprising nepenthesin or a derivative thereof and methods of using nepenthesin or a derivative thereof to treat bacterial infections of the gastrointestinal tract, such as C. difficile or H. pylori. Further provided herein are compositions comprising recombinant nepenthesin I or nepenthesin II, or homologous proteins, and methods for making the same.

The Cloning and Expression of Comosain's Minigene and the Methods and Compositions for treating and/or preventing various types of cancer which comprise administered an effective amount of Glyco-polypeptides and Bioflavonoids such as Comosain, Ananase, Bromelainases, Trypsin, Pepsin, Quercetin, Rutin, Naringenin, Genistein, Hesperetin, etc and/or a mixture thereof.