A method of preparing a microorganisms-immobilized felt-based resin includes the following steps: providing a mixture of an acrylate monomer, an initiator, a solvent, and water; adding a felt to the mixture; initiating a polymerization reaction of the mixture to form a felt-based resin; and immobilizing microorganisms on the felt-based resin to form the microorganisms-immobilized felt-based resin.

This document relates to materials and methods for using controlled radical polymerization (e.g., atom transfer radical polymerization) to generate protein-polymer conjugates in which two or more polymer molecules are attached to individual initiator molecules on the protein.

This document relates to materials and methods for using controlled radical polymerization (e.g., atom transfer radical polymerization) to generate protein-polymer conjugates in which two or more polymer molecules are attached to individual initiator molecules on the protein.

The invention provides a transaminase mutant and application thereof, wherein the amino acid sequence of the transaminase mutant is formed after mutation of the amino acid sequence as shown in SEQ ID NO: 1, and mutated amino acid sites comprise T7C+S47C sites. The transaminase mutant having the mutation sites can be further prepared into an immobilized enzyme through an immobilization technology, the immobilized enzyme has relatively high activity and high stability, can be recycled for multiple times, and is applicable to continuous flow reaction in a packed bed.

Various embodiments are described herein for the fabrication enzyme degradable hydrogels useful as payload delivery systems. More particularly, embodiments disclosed herein relate to enzyme-degradable hydrogel systems comprising a crosslinkable polymer, such as a chemically-modified biopolymer, for example, chemically-modified gelatin, the hydrogel formed by a method comprising sequential physical and chemical crosslinking steps, for delivery of various payloads. Enzymes may be selected and administered to tune the release profile of the hydrogel. The payload can be, but not limited to, drugs, markers, cells, or these members encapsulated within another drug delivery such as a nanoparticle, or liposome. The hydrogel system can also be combined with another device such as a contact lens or bandage for wound healing.

A stimulus-responsive carrier, a method for making and a method of using the same are disclosed. The stimulus-responsive carrier comprises a polymeric component comprising poly(N-isopropylacrylamide) (PNIPAM), a copolymer comprising units derived from N-isopropylacrylamide and acrylic acid (PNIPAM-AA), poly N-vinylpyrrolidone, a copolymer of N-isopropylacrylamide and hydroxymethylacrylamide (PNIPAM-HMAAm), a copolymer of N-isopropylacrylamide and allylamine (poly(NIPAAM-co-allylamine)), poly 2-(2-methoxyethoxy) ethyl methacrylate, or any combination thereof; and a second component disposed within the polymeric component, the second component comprising a hydrogel, wherein the second component has a different composition than the polymeric component. The stimulus-responsive carrier is responsive to a stimulus comprising a temperature change, a pH change, application of a magnetic field, or any combination thereof.

A stimulus-responsive carrier, a method for making and a method of using the same are disclosed. The stimulus-responsive carrier comprises a polymeric component comprising poly(N-isopropylacrylamide) (PNIPAM), a copolymer comprising units derived from N-isopropylacrylamide and acrylic acid (PNIPAM-AA), poly N-vinylpyrrolidone, a copolymer of N-isopropylacrylamide and hydroxymethylacrylamide (PNIPAM-HMAAm), a copolymer of N-isopropylacrylamide and allylamine (poly(NIPAAM-co-allylamine)), poly 2-(2-methoxyethoxy) ethyl methacrylate, or any combination thereof; and a second component disposed within the polymeric component, the second component comprising a hydrogel, wherein the second component has a different composition than the polymeric component. The stimulus-responsive carrier is responsive to a stimulus comprising a temperature change, a pH change, application of a magnetic field, or any combination thereof.

The present invention relates to an enzyme-catalyzed process for producing UDP-galactose from low-cost substrates uridine monophosphate and D-galactose in a single reaction mixture. The process can be operated (semi)continuously or in batch mode. The process can be extended to uridine as starting material instead of uridine monophosphate. Further, the process can be adapted to produce galactosylated molecules and biomolecules including saccharides, proteins, peptides, glycoproteins or glycopeptides, particularly human milk oligosaccharides (HMO) and (monoclonal) antibodies.

The present invention relates to an enzyme-catalyzed process for producing UDP-galactose from low-cost substrates uridine monophosphate and D-galactose in a single reaction mixture. The process can be operated (semi)continuously or in batch mode. The process can be extended to uridine as starting material instead of uridine monophosphate. Further, the process can be adapted to produce galactosylated molecules and biomolecules including saccharides, proteins, peptides, glycoproteins or glycopeptides, particularly human milk oligosaccharides (HMO) and (monoclonal) antibodies.

The present invention is directed to synthesizing and using fluid-insoluble material complexes that capture biologicals and remove them from samples in microscopic scale fluids, such as in droplets, wells, and micro-wells. The present invention also pertains to the option of detecting the captured biologicals, to the option of modifying the captured biologicals, and to the option of controllably releasing the captured biologicals.