The present inventions in various aspects provide elastic biodegradable polymers. In various embodiments, the polymers are formed by the reaction of a multifunctional alcohol or ether and a difunctional or higher order acid to form a pre-polymer, which is cross-linked to form the elastic biodegradable polymer. In preferred embodiments, the cross-linking is performed by functionalization of one or more OR groups on the pre-polymer backbone with vinyl, followed by photopolymerization to form the elastic biodegradable polymer composition or material. Preferably, acrylate is used to add one or more vinyls to the backbone of the pre-polymer to form an acrylated pre-polymer. In various embodiments, acrylated pre-polymers are co-polymerized with one or more acrylated co-polymers.

The invention relates to an immobilized protein material comprising a protein that is immobilized on a glass material or organic polymer through affinity tag binding. The glass material may be a porous glass material such as (hybrid) controlled porosity glass. The invention also relates to the use of an immobilized enzyme material as a heterogeneous biocatalyst in chemical synthesis. The invention further relates to a method for the immobilization of affinity tagged proteins on a glass material or organic polymer, and to a method for the purification and isolation of affinity tagged proteins by the immobilization of such proteins on a glass material or organic polymer.

In various embodiments methods of preparing hydroxybutyryl 3-hydroxybutyrate and related compounds are provided along with methods of use thereof.

The present disclosure relates to an electrode system for measuring the concentration of an analyte under in-vivo conditions, comprising an electrode with immobilized enzyme molecules and a diffusion barrier that controls diffusion of the analyte from body fluid surrounding the electrode system to the enzyme molecules.

The present disclosure relates to an electrode system for measuring the concentration of an analyte under in-vivo conditions, comprising an electrode with immobilized enzyme molecules and a diffusion barrier that controls diffusion of the analyte from body fluid surrounding the electrode system to the enzyme molecules.

The invention provides a transaminase mutant and application thereof, wherein the amino acid sequence of the transaminase mutant is formed after mutation of the amino acid sequence as shown in SEQ ID NO: 1, and mutated amino acid sites comprise T7C+S47C sites. The transaminase mutant having the mutation sites can be further prepared into an immobilized enzyme through an immobilization technology, the immobilized enzyme has relatively high activity and high stability, can be recycled for multiple times, and is applicable to continuous flow reaction in a packed bed.

The present invention provides a method for preparing salidroside. The present invention uses -glucoside and CoFe.sub.2O.sub.4 particles to form a cross-linked aggregate capable of effectively catalyzing the reaction of -D-ghucose and tyrosol, thereby increasing the yield of the salidroside. The steps of the preparation method of the present invention are simple and short, and the method is easy to operate and readily applicable to industrial production.

The present invention provides a method for preparing salidroside. The present invention uses -glucoside and CoFe.sub.2O.sub.4 particles to form a cross-linked aggregate capable of effectively catalyzing the reaction of -D-ghucose and tyrosol, thereby increasing the yield of the salidroside. The steps of the preparation method of the present invention are simple and short, and the method is easy to operate and readily applicable to industrial production.

The invention concerns a method for depositing nano-objects on the surface of a gel comprising a polymer matrix having at least two contiguous zones of distinct rigidities, said method comprising the steps of: a) providing a gel comprising a polymer matrix and a solvent within the polymer matrix, the polymer matrix forming a three-dimensional array capable of swelling in the presence of said solvent, wherein the solubility of the polymer matrix, at 1 bar and 25 C., in the solvent is less than 1 g/L, the polymer matrix comprising at least two contiguous zones of distinct rigidities having a rigidity gradient greater than or equal to 0.1 kPa/m, then b) depositing nano-objects on the surface of the gel, then c) evaporating the solvent from the gel at least until the variation of the rate of evaporation of the solvent from the at least one least rigid zone of the gel is not constant over time, by which the nano-objects migrate towards the at least one most rigid zone of the gel and a gel is obtained for which the surface is at least partially coated with nano-objects, and wherein the density per unit area of nano-objects of the at least one most-rigid zone among the at least two contiguous zones is greater than that of the at least one least-rigid zone among the at least two contiguous zones, the gel that can be obtained and its applications.

The invention concerns a method for depositing nano-objects on the surface of a gel comprising a polymer matrix having at least two contiguous zones of distinct rigidities, said method comprising the steps of: a) providing a gel comprising a polymer matrix and a solvent within the polymer matrix, the polymer matrix forming a three-dimensional array capable of swelling in the presence of said solvent, wherein the solubility of the polymer matrix, at 1 bar and 25 C., in the solvent is less than 1 g/L, the polymer matrix comprising at least two contiguous zones of distinct rigidities having a rigidity gradient greater than or equal to 0.1 kPa/m, then b) depositing nano-objects on the surface of the gel, then c) evaporating the solvent from the gel at least until the variation of the rate of evaporation of the solvent from the at least one least rigid zone of the gel is not constant over time, by which the nano-objects migrate towards the at least one most rigid zone of the gel and a gel is obtained for which the surface is at least partially coated with nano-objects, and wherein the density per unit area of nano-objects of the at least one most-rigid zone among the at least two contiguous zones is greater than that of the at least one least-rigid zone among the at least two contiguous zones, the gel that can be obtained and its applications.