Provided are combinations, compositions and kits containing a hyaluronan degrading enzyme, such as a soluble hyaluronidase, for treatment of hyaluronan-associated conditions, diseases and disorders. In one example, the products include an additional agent or treatment. Such products can be used in methods for administering the products to treat the hyaluronan-associated diseases and conditions, for example, hyaluronan-associated cancers, for example, hyaluronan-rich tumors. The methods include administration of the hyaluronan degrading enzyme composition alone or in combination with other treatments. Also provided are methods and compositions for providing sustained treatment effects in hyaluronan-associated diseases and conditions.

Provided is a means capable of measuring an analyte concentration with high accuracy without requiring fractionation of a biological component. The present invention relates to a method for measuring an analyte concentration in a specimen, including (1) preparing the specimen, (2) mixing a coloring reagent, an oxidoreductase, and a light-scattering adjustment reagent with the specimen to obtain an analysis sample having substantially the same scattered light as the specimen, and (3) measuring the analyte concentration by using the analysis sample.

Materials and methods for generating protein-polymer conjugates on solid supports are provided herein. The methods can include, for example, reversibly immobilizing a protein on a solid support, modifying the protein by adding polymer subunits, and releasing the protein-polymer conjugate from the solid support.

The present invention relates to a process for preparing a polymer/biological entities alloy, comprising a step of mixing a polymer and biological entities that degrade it, during a heat treatment, said heat treatment being performed at a temperature T above room temperature and said biological entities being resistant to said temperature T, characterized in that said biological entities are chosen from enzymes that degrade said polymer and microorganisms that degrade said polymer.

A material for reducing the content of polluting and/or unwanted substances in a liquid includes at least one polymeric and/or inorganic base, suitable to be immersed in the liquid, having at least a surface, whereto is bonded at least one catalyst of the degradation reaction of inorganic salts in gaseous substances. The catalyst, whose function is to reduce the energy required for the activation of said reaction and to accelerate its progress, is an enzyme preferably selected among the lyase group, the hydrolase group and the oxidoreductase group.

Biocatalysts disclosed herein can have a core and a shell, with the core including a polymer having a pyridine functional group, and the shell including an enzyme that interacts with the polymer. The biocatalysts disclosed herein can have improved stability, control, and activity as compared to the enzyme in a free, non-interacted state.

Biocatalysts disclosed herein can have a core and a shell, with the core including a polymer having a pyridine functional group, and the shell including an enzyme that interacts with the polymer. The biocatalysts disclosed herein can have improved stability, control, and activity as compared to the enzyme in a free, non-interacted state.

Inventive concepts relate generally to nanofibrous scaffolds useful for electrophysiological assays. Scaffolds include polymeric nanofibrous components and electrically excitable cells immobilized at a distinct cell seeding domains on the scaffold. Methods and kits including the scaffolds are also described.

Embodiments of the present disclosure provide for nanozymes that can include a therapeutic agent, methods of making nanozymes, methods of using nanozymes, and the like. In some embodiments, the nanozymes can include a shell that can surround a hollow core that can be configured to receive a compound and the shell can include a recognition moiety and an enzyme.

The object of the present invention is to provide a cell mass or cell structure-embedding agent that can stably transport cell masses or cell structures in a case of transportation at a low temperature and after transportation, conveniently recover a cell mass or cell structure from the cell mass or cell structure-embedding agent, a cell mass or cell structure-containing composition, and a kit. According to the present invention, provided is a cell mass or cell structure-embedding agent including: polypeptide which is represented by Formula 1 and in which an area of the maximum molecular weight peak in molecular weight distribution measurement is 80% or more of the total area of all of the molecular weight peaks.

A-[(Gly-X-Y).sub.n].sub.m-BFormula (1):

In the formula, X's and Y's each independently represent an amino acid, m is an integer of 2 to 10, n is an integer of 3 to 100, and A and B represent any amino acids or amino acid sequences.