Provided is a method for collecting RNA contained in skin surface lipids (SSL). A method for preparing SSL-derived RNA. In this method, an aqueous solution containing RNA prepared from SSL of a subject is mixed with a water-soluble organic solvent, and the resulting mixture is allowed to contact with a solid phase material to separate RNA. By adjusting the concentration of the water-soluble organic solvent in the mixture, the yield of RNA improves.

Provided herein are devices, systems, kits and methods for predicting or determining the gender of a fetus using cell free fetal nucleic acids in a small amount of maternal biological sample. Devices can be used at point of need during early stages of pregnancy and are compatible with communication devices.

A nucleic acid extraction apparatus having a workbench having a table top, and further comprises a sliding seat, a sample rack, a reagent strip rack, a pipetting table, a piston assembly and a suction assembly. The nucleic acid extraction apparatus comprises the sliding seat and the pipetting table, the sample rack and the reagent strip rack are arranged on the sliding seat, the piston assembly and the suction assembly are respectively arranged on the pipetting table, the sample rack is provided with sample holes, the reagent strip rack is provided with accommodation slots, the number of accommodation slots corresponds to that of the sample holes on a one-to-one basis, and when reagent strips are respectively accommodated in the accommodation slots, a first suction head hole, second suction head holes and various reagent holes in each reagent strip respectively correspond to the corresponding sample holes.

Provided herein, in some embodiments, are methods, compositions and kits for large-scale production of long single-stranded DNA in solution.

Provided is a syringe-type nucleic acid extraction apparatus, a nucleic acid extraction kit capable of simply and quickly separating nucleic acid without the aid of electronic devices or other mechanical devices, and a method for extracting the nucleic acid. According to the present disclosure, the nucleic acid extraction apparatus includes a syringe contacting an upper surface of the nucleic acid purification filter and including a cylinder and a piston; a tip having an upper surface in contact with a lower surface of the nucleic acid purification filter and a lower side through which washing liquid or a nucleic acid elution buffer moves; and a coupler configured to connect a lower end of the syringe to the upper end of the tip and into which the nucleic acid purification filter is inserted.

A device for isolating DNA from a sample containing cells, including a cartridge having an entrance port and an exit port, a membrane disposed between the entrance port and the exit port, and a plurality of channels between the membrane and the exit port. Additionally, systems and methods for isolating DNA from a sample containing cells and also systems and methods for amplifying and isolating single-stranded DNA from a sample containing DNA.

Disclosed are an automated nucleic acid extraction method and device. The device includes a base body, a cassette, a driving unit, a moving frame and a syringe. The base body comprises a sample accommodating area, a column accommodating area, a cassette accommodating area and a collection tube being arranged in a linear direction. The cassette is arranged in the cassette accommodating area and includes two parallel walls and at least two vertical walls. The parallel walls and the vertical walls jointly form a lysis buffer well, at least one wash buffer well and an elution buffer well. Each vertical wall includes a load-bearing abutment. The driving unit and the moving frame are arranged on the base body. The syringe is arranged on the moving frame and is driven by the driving unit to reciprocate along with the moving frame in the linear direction.

A device and method for isolating extracellular vesicles from biofluids is disclosed. A nanoporous silicon nitride membrane is provided with a tangential flow of biofluid. A pressure gradient through the nanoporous silicon nitride membrane facilitates capture of extracellular vesicles from the tangential flow vector of biofluid. Reversal of the pressure gradient results in the release of the extracellular vesicles for subsequent collection.

There is provided an impurity precipitant composition used for precipitating and removing impurities that inhibit the pure separation of DNA during the process of extracting DNA by lysis of cells in a sample. The composition includes 1.3-2.1% (w/v) phosphotungstic acid hydrate, 1.2-1.8% (w/v) zinc acetate dihydrate, 13.0-19.0% (v/v) acetic acid, with the remainder being water.

A method for preparing test solution for pathogen detection purpose, system, kit, detection primer and method are provided. The method of preparing a test solution includes lysing the sample to be tested with a lysis buffer to release the nucleic acids contained in the sample to obtain a lysis buffer containing nucleic acids and/or pathogen nucleic acids; extracting the lysis buffer containing nucleic acids through a nucleic acid extraction device to obtain an extract containing host nucleic acids and/or pathogen nucleic acids; preparing the test solution for pathogen detection purposes from the extract. A large volume of samples can be used in the present application and it greatly improves sensitivity and specificity of the assay.