A method for developing capture agents for target proteins employs a compound library to find cyclic peptide sequences that bind the target protein. The target protein is also reacted with a clickable group-provider reagent to provide the protein with clickable groups. The compounds in the library are provided with complementary clickable groups that bind the clickable group on the target protein when the peptide sequences bind the target protein. In some embodiments, the cyclic peptide sequences that bind the target protein are incorporated into constructs having one or more arms that can serve as capture agents or potential treatments against the pathogens from which the target protein is derived. Some embodiments provide pharmaceutical compositions for immunoassays, diagnostics, therapeutics or the like, that employ the constructs.

Provided herein are methods and composition for processing samples that contain nucleic acids, or cells containing nucleic acids, of a microbiome, using amounts of primers within a range of mole values and rounds of polymerase chain reaction (PCR) within a range of numbers of rounds.

A device for the storage and/or the editing of digital data including at least one double stranded, replicative, composite nucleic acid molecule. The composite nucleic acid molecule includes both digital data-encoding and non-digital data-encoding nucleic acids. The non-digital data-encoding nucleic acids may allow indexing and/or the provision of metadata for the flanking digital data-encoding nucleic acid. The composite nucleic acid molecules may be pooled to constitute an array and arrays may constitute a DNA drive, which represents the physical support on which the digital data are stored.

Methods of normalizing two or more nucleic acid samples are provided. Aspects of the methods include contacting each of the two or more nucleic acid samples with a limiting amount of a target binding moiety that specifically binds to a common target in each of the two or more nucleic acid samples to produce binding complexes in each of the two or more nucleic acid samples; and separating the binding complexes from unbound nucleic acids in each of the two or more nucleic acid samples to normalize the two or more nucleic acid samples. Compositions and kits for use in performing the methods are also provided.

The present disclosure provides compositions, methods, systems, and devices for polynucleotide processing. Such polynucleotide processing may be useful for a variety of applications, including polynucleotide sequencing.

The present invention discloses a profiling technique for a target molecule population in a sample including an unknown target molecule, using an aptamer. In the method of the present invention, the target molecule population in the sample may be provided as an aptamer profile including an unknown target molecule, and this aptamer profile can be used to determine whether drug prescription is appropriate (i.e., anticancer drug companion diagnosis, etc.), to provide disease diagnosis information, to monitor drug treatment, to determine drug compliance, to determine the degree or absence/presence of in vitro cellular response to drug treatment, and to obtain useful information to humans for classification or identification of species, etc.

The present disclosure provides compositions, methods, systems, and devices for polynucleotide processing. Such polynucleotide processing may be useful for a variety of applications, including polynucleotide sequencing.

Provided herein are encoded split pool libraries useful, inter alia, for forming highly diverse and dense arrays for screening and detection of a variety of molecules.

The present invention provides a poly(alkylene oxide) polymer based size selective nucleic acid enrichment method for enriching target nucleic acid molecules from a nucleic acid containing sample which comprises target nucleic acid molecules and non-target nucleic acid molecules, wherein the target nucleic acid molecules are longer than the non-target nucleic acid molecules, the method comprising (a) preparing a binding mixture comprising—the nucleic acid containing sample, —a poly(alkylene oxide) polymer and—a salt and binding nucleic acid molecules to a solid phase which comprises a functional group, preferably carboxylated magnetic particles, wherein the bound nucleic acid molecules comprise target nucleic acid molecules; (b) preferably separating the solid phase with the bound nucleic acid molecules from the remaining sample; (c) contacting the solid phase with the bound nucleic acid molecules at least once with a reagent composition comprising a poly(alkylene oxide) polymer and a salt to selectively elute non-target nucleic acid molecules, wherein the concentration (w/v) of the poly(alkylene oxide) polymer in the reagent composition of step (c) is lower than the concentration (w/v) of the poly(alkylene oxide) polymer in the binding mixture of step (a); (d) optionally washing the bound target nucleic acid molecules; and (e) eluting the bound target nucleic acid molecules from the solid phase. Said method allows the size selective purification of target DNA molecules and is particularly suitable for sequencing applications. It is more time- and cost efficient than prior art methods and provides excellent purification results. Moreover, further methods and kits are provided.

The present disclosure relates to cell populations and systems for detection of compounds in an environment. Specifically the disclosure relates to methods for generating reproducible genome-wide edited populations of microbes that display novel, defined, and reproducible phenotypes when exposed to one or more chemicals. In some applications, such phenotypes are read out by barcode amplicon and compared against population fingerprints. In other applications digital information is stored in such populations of microorganisms. The digital information can be retrieved from the microorganisms with Boolean logic.