Provided herein are monoliths with attached recognition compounds which selectively bind ligands, methods of preparing such monoliths, arrays thereof and uses thereof. For example, monoliths provide herein can be used in columns and arrays thereof.

This disclosure describes improvements to both hardware and enzymatic reactions used in single cell analyses such as but not limited to Seq-well that enable significant increases in the yield of transcripts per cell, improved portability and ease of use, increased scalability of the assay, and linkage of transcript information to other measurements made in the picowell arrays.

The present invention relates to compositions and methods for material characterization using probe libraries. The methods include steps of unbiased sensing with probe libraries (e.g., oligonucleotide probes, or conjugated probes that comprise peptides conjugated to polynucleotide barcodes) that are followed with identifying informative patterns of the bounded probes when the probed libraries are applied to a material. In some examples, the probes used herein comprise binding moieties composed of random sequences (e.g., random peptide sequences or random polynucleotide sequences) that enable the unbiased sensing of the substrates in a sample for the determination of known and unknown materials. In some examples, the methods herein further comprise analyzing the sequences of the bounded oligonucleotide probes or the bounded conjugated probes and determining the patterns of the sequences that is indicative of the substrates bounded by the probes.

An apparatus and method for applying a chemistry to samples of interest are provided. The apparatus and method include a flexible tape mounted on an arrangement of guide rollers. Samples of interest (e.g., clusters of DNA templates) are bound to at least one surface of the flexible tape. The method and apparatus further comprise one or more read heads in relation to the flexible tape and a plurality of reservoirs along a path of the flexible tape. The reservoirs comprise liquids comprising chemical reagents for performing the chemistry on the samples of interest bound to the at least one surface of the flexible tape. The method and apparatus further comprise a drive system for driving at least one of the guide rollers to advance the flexible tape into and out of the reservoirs.

Nucleic acid-guided ordered protein assembly (NOPA) arrays and methods for their generation and related applications are disclosed herein.

This application provides a bead with a covalently attached chemical compound and a covalently attached DNA barcode and methods for using such beads. The bead has many substantially identical copies of the chemical compound and many substantially identical copies of the DNA barcode. The compound consists of one or more chemical monomers, where the DNA barcode takes the form of barcode modules, where each module corresponds to and allows identification of a corresponding chemical monomer. The nucleic acid barcode can have a concatenated structure or an orthogonal structure. Provided are method for sequencing the bead-bound nucleic acid barcode, for cleaving the compound from the bead, and for assessing biological activity of the released compound.

The present invention discloses a construction method for serial sequencing libraries of RAD tags, including the following steps: conducting an enzyme digestion reaction with DNA using endonuclease; ligating; enzyme-digested fragments with adaptors that contain restriction enzyme sites of SapI, featured base sequences for serial ligation of RAD tags, and universal sequences for the binding of amplification primers; conducting PCR amplification using a combination of biotin primers and general primers; collecting target PCR product by gel; amplifying again; and equally mixing and purifying; conducting enzyme digestion on the PCR products using the SapI enzyme and heating tags in series; purifying the long serial tags through gels and then conducting PCR. amplification using the barcode primers for constructing the libraries; and library sequencing, The present invention can be applied to the screening and detection of genome-wide genetic markers and epigenetic variations at high throughput and low cost

An object of the present invention is to provide a method of stably introducing a heterocycle into a substrate peptide by using an azoline backbone introducing enzyme. The present invention provides a method of introducing a heterocycle into a leader-sequence-free substrate peptide by using an azoline backbone introducing enzyme to which a leader sequence of the substrate has been bound.

The present invention generally relates to a method which makes use of electrowetting to automate PCR amplification wherein the PCR reagents are contained in droplets, and further wherein the quantity of PCR product is monitored in real time thus enabling stopping amplification once a desired quantity of PCR product has been generated.

This disclosure describes improvements to both hardware and enzymatic reactions used in single cell analyses such as but not limited to Seq-well that enable significant increases in the yield of transcripts per cell, improved portability and case of use, increased scalability of the assay, and linkage of transcript information to other measurements made in the picowell arrays.