Disclosed are methods and kits for isolating nucleic acids having a size above a desired cut-off size from a nucleic acid containing sample. The method comprises combining the sample with a binding buffer, alcohol and silicon carbide to provide a binding mixture. Nucleic acids having a size above the desired cut-off size are selectively bound to the silicon carbide. The cut-off size for selective binding to the silicon carbide is determined by the alcohol concentration of the binding mixture. The bound nucleic acids are separated from the remaining sample. The bound nucleic acids are optionally washed and then eluted from the silicon carbide. The kit comprises a buffer binding to be diluted with alcohol to provide an alcohol concentration of about 1 to about 50% (v/v), a wash solution, an elution solution, silicon carbide and instructions for adjusting the alcohol concentration to selectively bind nucleic acids having a size above the desired cut-off size.

Disclosed are methods and kits for isolating nucleic acids having a size above a desired cut-off size from a nucleic acid containing sample. The method comprises combining the sample with a binding buffer, alcohol and silicon carbide to provide a binding mixture. Nucleic acids having a size above the desired cut-off size are selectively bound to the silicon carbide. The cut-off size for selective binding to the silicon carbide is determined by the alcohol concentration of the binding mixture. The bound nucleic acids are separated from the remaining sample. The bound nucleic acids are optionally washed and then eluted from the silicon carbide. The kit comprises a buffer binding to be diluted with alcohol to provide an alcohol concentration of about 1 to about 50% (v/v), a wash solution, an elution solution, silicon carbide and instructions for adjusting the alcohol concentration to selectively bind nucleic acids having a size above the desired cut-off size.

Nosocomial infections are a major threat to the health sector. A specific category of these are the infections caused by antibiotic induced susceptibility to various pathogenic bacteria. This disclosure relates generally to method and system for combating infections due to antibiotic induced pathogens. The system provides strategies to combat pathogenic infections caused by multi-drug resistant (MDR) and extensively drug resistant (XDR) strains of antibiotic induced pathogens. The idea used in this disclosure utilizes the fact that multiple occurrences of a conserved stretch of nucleotide sequence on a pathogen genome and surrounded by genes encoding virulence factors or which are in vicinity of genes essential for survival of the candidate pathogen can be targeted to disrupt the overall genetic machinery of the pathogen. The present disclosure has been explained on sequenced genomes of Clostridium difficile and vancomycin-resistant Enterococcus sp.

Compositions and methods for inducing and isolating virus-like particles (VLPs), and for allowing real-time assessment of VLP-captured analytes obtained from targeted living mammalian cells, are provided.

Compositions and methods for inducing and isolating virus-like particles (VLPs), and for allowing real-time assessment of VLP-captured analytes obtained from targeted living mammalian cells, are provided.

The present invention relates to a library of particles, the library displaying a plurality of different T cell receptors (TCRs), wherein the plurality of TCRs consists essentially of TCRs comprising an alpha chain comprising an alpha chain variable domain and a beta chain comprising a beta chain variable domain and the library comprises more than one TRAV gene product and/or more than one TRBV gene product, wherein the beta chain variable domain does not comprise one or more of a TRBV5-1, 5-3, 5-4, 5-5, 5-6, 5-7 or 5-8 gene product and wherein the plurality of TCRs do not consist essentially of TCRs comprising a TRAV12-2 gene product from a natural repertoire and a TRBV6 gene product from a natural repertoire and TCRs comprising a TRAV21 gene product from a natural repertoire and a TRBV6 gene product from a natural repertoire.

The present disclosure is concerned with compositions, methods, and kits for preparing a sequencing library. In one embodiment, methods include producing a library of target nucleic acids having the same adapter at each end and then switching the identity of one adapter to result in target nucleic acids flanked by distinct adapters.

The present invention is a method and compositions for forming a library for nucleic acids sequencing simultaneously from DNA and RNA present in a sample.

The present invention is a method and compositions for forming a library for nucleic acids sequencing simultaneously from DNA and RNA present in a sample.

Single cell analysis from tumor tissue comprising tumor cells and immune competent cells and from peripheral white blood cells are used to obtain an immunome signature, and to gain information about the TCR repertoire. Such information is then employed to generate recombinant and patient specific therapeutic cells, including T cells (including T effector memory, T memory stem, naïve T, T central memory, CD8+ T, and CD4+ T cells), NK cells (cord-blood derived or PBMC derived or NK92), NKT cells, and dendritic cells.