The present disclosure provides instruments, modules and methods for improved detection of edited cells following nucleic acid-guided nuclease genome editing. The disclosure provides improved automated instruments that perform methodsincluding high throughput methodsfor screening cells that have been subjected to editing and identifying cells that have been properly edited.

A method for characterizing toxicity of toxic pollutants and a method for characterizing comprehensive toxicity of water bodies. The methods include constructing a reporter gene cell line expressing CHOP gene associated with endoplasmic reticulum stress.

A method for characterizing toxicity of toxic pollutants and a method for characterizing comprehensive toxicity of water bodies. The methods include constructing a reporter gene cell line expressing CHOP gene associated with endoplasmic reticulum stress.

The present disclosure provides instruments, modules and methods for improved detection of edited cells following nucleic acid-guided nuclease genome editing. The disclosure provides improved automated instruments that perform methodsincluding high throughput methodsfor screening cells that have been subjected to editing and identifying cells that have been properly edited.

The present disclosure provides instruments, modules and methods for improved detection of edited cells following nucleic acid-guided nuclease genome editing. The disclosure provides improved automated instruments that perform methodsincluding high throughput methodsfor screening cells that have been subjected to editing and identifying cells that have been properly edited.

The invention relates to a method for preparative production of long nucleic acids by PCR. The method involves the following hybridization steps: a) a nucleic acid base sequence is hybridized on the 3 and 5 ends with an adapter primer; b) the product from step a) is hybridized on the 3 and 5 ends with an extension primer containing an extension sequence, wherein a nucleic acid with extension sequences amplified and enlarged in the 3 and 5 ends of the nucleic acid base sequence is then formed from the nucleic acid base sequence. The invention also relates to different applications of the inventive method.

The invention relates to a method for preparative production of long nucleic acids by PCR. The method involves the following hybridization steps: a) a nucleic acid base sequence is hybridized on the 3 and 5 ends with an adapter primer; b) the product from step a) is hybridized on the 3 and 5 ends with an extension primer containing an extension sequence, wherein a nucleic acid with extension sequences amplified and enlarged in the 3 and 5 ends of the nucleic acid base sequence is then formed from the nucleic acid base sequence. The invention also relates to different applications of the inventive method.

The invention relates to an artificial nucleic acid molecule comprising at least one open reading frame and at least one 3-untranslated region element (3-UTR element) and/or at least one 5-untranslated region element (5-UTR element), wherein the at least one 3-UTR element and/or the at least one 5-UTR element prolongs and/or increases protein production from said artificial nucleic acid molecule and wherein the at least one 3-UTR element and/or the at least one 5-UTR element is derived from a stable mRNA. The invention further relates to the use of such an artificial nucleic acid molecule in gene therapy and/or genetic vaccination. Furthermore, methods for identifying a 3-UTR element and/or a 5-UTR derived from a stable mRNA element are disclosed.

The invention relates to an artificial nucleic acid molecule comprising at least one open reading frame and at least one 3-untranslated region element (3-UTR element) and/or at least one 5-untranslated region element (5-UTR element), wherein the at least one 3-UTR element and/or the at least one 5-UTR element prolongs and/or increases protein production from said artificial nucleic acid molecule and wherein the at least one 3-UTR element and/or the at least one 5-UTR element is derived from a stable mRNA. The invention further relates to the use of such an artificial nucleic acid molecule in gene therapy and/or genetic vaccination. Furthermore, methods for identifying a 3-UTR element and/or a 5-UTR derived from a stable mRNA element are disclosed.

Methods of redirecting carbon flux and increasing C2/C3 or a C4/5/6 carbon chain length carbon-based chemical product yield in an organism, nonnaturally occurring organisms with redirected carbon flux and increased C2/C3 or C4/5/6 carbon chain length carbon-based chemical product yield and methods for using these organisms in production of C2/C3 or C4/5/6 carbon chain length carbon-based chemical products are provided.