The present disclosure provides instruments, modules and methods for improved detection of edited cells following nucleic acid-guided nuclease genome editing. The disclosure provides improved automated instruments that perform methods—including high throughput methods—for screening cells that have been subjected to editing and identifying cells that have been properly edited.

The present disclosure provides instruments, modules and methods for improved detection of edited cells following nucleic acid-guided nuclease genome editing. The disclosure provides improved automated instruments that perform methods—including high throughput methods—for screening cells that have been subjected to editing and identifying cells that have been properly edited.

The present disclosure provides instruments, modules and methods for improved detection of edited cells following nucleic acid-guided nuclease genome editing. The disclosure provides improved automated instruments that perform methods—including high throughput methods—for screening cells that have been subjected to editing and identifying cells that have been properly edited.

The present disclosure provides instruments, modules and methods for improved detection of edited cells following nucleic acid-guided nuclease genome editing. The disclosure provides improved automated instruments that perform methodsincluding high throughput methodsfor screening cells that have been subjected to editing and identifying cells that have been properly edited.

The present invention provides a reversibly cross-linked hydrogel comprising a sample, wherein the sample comprises extracellular nucleic acid. Corresponding methods of preparing, transporting and/or storing the nucleic acid-containing hydrogel, as well as uses thereof, are also provided herein.

The present invention provides a reversibly cross-linked hydrogel comprising a sample, wherein the sample comprises extracellular nucleic acid. Corresponding methods of preparing, transporting and/or storing the nucleic acid-containing hydrogel, as well as uses thereof, are also provided herein.

The present invention relates to methods of enhancing the translation ability and stability of an RNA molecule. The methods involve providing a cell-free composition comprising an RNA molecule to be translated, where the RNA molecule lacks an N.sup.6,2O-dimethyladenosine (m.sup.6A.sub.m) residue. Also disclosed are methods of making RNA molecules and treatment methods using an RNA molecule comprising a 7-methylguanosine (m.sup.7G), a 5 triphosphate linker (-ppp-), and an N.sup.6,2-0-dimethyladenosine (m.sup.6A.sub.m).

The present invention relates to methods of enhancing the translation ability and stability of an RNA molecule. The methods involve providing a cell-free composition comprising an RNA molecule to be translated, where the RNA molecule lacks an N.sup.6,2O-dimethyladenosine (m.sup.6A.sub.m) residue. Also disclosed are methods of making RNA molecules and treatment methods using an RNA molecule comprising a 7-methylguanosine (m.sup.7G), a 5 triphosphate linker (-ppp-), and an N.sup.6,2-0-dimethyladenosine (m.sup.6A.sub.m).

The present disclosure provides instruments, modules and methods for improved detection of edited cells following nucleic acid-guided nuclease genome editing. The disclosure provides improved automated instruments that perform methodsincluding high throughput methodsfor screening cells that have been subjected to editing and identifying cells that have been properly edited.

The present invention relates to nucleic acid molecules containing poly (dA:dT) regions which are stabilized in E.-coli, methods of propagating such nucleic acid molecules in E. coli, methods of obtaining RNA, peptides or proteins using such nucleic acid molecules and to RNA which is obtained from such nucleic acid molecules and its use. In particular, the poly (dA:dT) regions contain at least one disruption by a sequence not encoding a sequence solely composed of A residues.