The present disclosure comprises methods and compositions comprising a plant transforming bacterium of the Order Rhizobiales comprising conditional negative selectable marker genes.

This invention relates to an artificial salt tolerant protein NLEA with the amino acid sequence shown in SEQ ID No.1 and a synthetic method of salt tolerant protein NLEA comprising the steps of retrieving different types of LEA proteins from LEA database; making multiple sequence alignment on different types of LEA proteins to obtain conserved short peptides; selecting hydrophilic short peptides with a hydrophilicity index higher than 3.5 from conserved short peptides; arranging and splicing hydrophilic short peptides in the order of isoelectric point size from large to small, to obtain salt tolerant protein NLEA. This invention involves bioinformatics analysis by retrieving different LEA conserved amino acid sequences of LEA protein data. Physical properties are analyzed to find short peptides of high hydrophilicity, and such short peptides are arranged in the order of isoelectric point size and spliced to get a new hydrophilic amino acid sequence.

The present disclosure relates to modified plant LysM receptor polypeptides with modified intracellular domains, specifically, modified ?A motifs and/or modified ?A motifs in the juxtamembrane domain. The present disclosure further relates to genetically modified plants including the modified plant LysM receptor polypeptides, and methods of producing the same.

A glycoside hydrolase, relating to the technical field of enzyme engineering/gene engineering. Provided are an enzyme of a GH112 glycoside hydrolase family, and an application of synthesizing human milk oligosaccharides (HMOs) by using an enzyme of a GH112 glycoside hydrolase family. A synthesis reaction is catalyzed by using the enzyme of the glycoside hydrolase family 112 (GH112) that is classified according to a Carbohydrate-Active-Enzymes (CAZy) database (http://www.cazy.org); and lacto-N-tetraose (LNT) is generated in an aqueous solution comprising lactose and/or galactose and/or galactose-1-phosphate and lacto-N-triose (LNTII). The enzyme of the glycoside hydrolase family 112 (GH112), and the preparation method therefor and the application thereof provided by the present invention have economic and social values.

A wheat variety designated A060013G1, the plants and seeds of wheat variety A060013G1, methods for producing a wheat plant produced by crossing the variety A060013G1 with another wheat plant, and hybrid wheat seeds and plants produced by crossing the variety A060013G1 with another wheat line or plant, and the creation of variants by backcrossing, mutagenesis or transformation of variety A060013G1 are disclosed. Methods for producing other wheat varieties or breeding lines derived from wheat variety A060013G1 and to wheat varieties or breeding lines produced by those methods are also provided.

Methods for producing single N-terminal domain functional camelid-derived heavy (H)-chain antibody fragments (VHH) that specifically bind to a receptor binding domain (RBD) of SARS-CoV-2 spike protein include transgenic plants, plant parts, plant cells, plant tissue, and seeds that express the VHH that specifically binds to an RBD of SARS-CoV-2 spike protein.

Agrobacterium strains that harbor transformation-enhancing genes on a plasmid capable of replication independently of the Agrobacterium chromosome, the Ti plasmid, and plant transformation binary vectors, and uses for these Agrobacterium strains are provided. Additionally, Agrobacterium strains that are deficient in DNA recombination functions that result in instability or rearrangement of plant transformation binary vectors, and that harbor transformation-enhancing genes on a plasmid capable of replication independently of the Agrobacterium chromosome, the Ti plasmid, and plant transformation binary vectors, and uses for these strains, are also provided. Further included are Agrobacterium strains that harbor transformation-enhancing genes integrated into the Agrobacterium chromosome at a locus that does not interfere with or otherwise compromise the normal growth and plant transformation ability of the Agrobacterium cells, and uses for these Agrobacterium strains. Plants made using these Agrobacterium strains are also described.

The present invention relates to Agrobacterium tumefaciens strains that comprise at least one deletion/mutation in a sequence selected from the group of IS426 copy I, IS426 copy II, the OriT-like sequence, and the border-like sequences, and their uses in safer and improved transformation procedures for cells.

Agrobacterium strains that harbor transformation-enhancing genes on a plasmid capable of replication independently of the Agrobacterium chromosome, the Ti plasmid, and plant transformation binary vectors, and uses for these Agrobacterium strains are provided. Additionally, Agrobacterium strains that are deficient in DNA recombination functions that result in instability or rearrangement of plant transformation binary vectors, and that harbor transformation-enhancing genes on a plasmid capable of replication independently of the Agrobacterium chromosome, the Ti plasmid, and plant transformation binary vectors, and uses for these strains, are also provided. Further included are Agrobacterium strains that harbor transformation-enhancing genes integrated into the Agrobacterium chromosome at a locus that does not interfere with or otherwise compromise the normal growth and plant transformation ability of the Agrobacterium cells, and uses for these Agrobacterium strains. Plants made using these Agrobacterium strains are also described.

Provided is a cucumber mosaic virus-based modified recombinant vector. The recombinant vector according to an example may significantly improve the efficiency of foreign protein expression in plants, and are more efficient at expressing a foreign protein than an existing recombinant vector, and thus may be widely utilized for the production of useful proteins in various plant species and for research thereon.