The invention relates to cells which make rhamnolipids and are genetically modified such that they have a decreased activity, compared to the wild type thereof, of a glucose dehydrogenase and to a method for producing rhamnolipids using the cells according to the invention.

Provided herein are methods and compositions for displaying a polypeptide on a tubular structure and uses of such displayed polypeptides in the production of antibodies or vaccines.

Provided herein are methods and compositions for displaying a polypeptide on a tubular structure and uses of such displayed polypeptides in the production of antibodies or vaccines.

Provided herein are methods for integrating a gene of interest into a chromosome of a host cell. In some embodiments, the methods include introducing into a host cell a first plasmid comprising a transposase coding sequence and a donor sequence, which includes a selectable marker coding sequence flanked by a first and a second lox site and is itself flanked by inverted repeats recognized by the transposase. Following transposase-mediated chromosomal integration of the donor sequence into the host cell, a second plasmid is introduced, which comprises the gene of interest and a second selectable marker coding sequence, both flanked by a first and a second lox site. The gene of interest is chromosomally integrated into the host cell by recombinase-mediated cassette exchange (RMCE) between the donor sequence and the second plasmid via Cre-/cuc recombination. Further provided herein are host cells, vectors, and methods of producing a product related thereto.

Provided herein are methods for integrating a gene of interest into a chromosome of a host cell. In some embodiments, the methods include introducing into a host cell a first plasmid comprising a transposase coding sequence and a donor sequence, which includes a selectable marker coding sequence flanked by a first and a second lox site and is itself flanked by inverted repeats recognized by the transposase. Following transposase-mediated chromosomal integration of the donor sequence into the host cell, a second plasmid is introduced, which comprises the gene of interest and a second selectable marker coding sequence, both flanked by a first and a second lox site. The gene of interest is chromosomally integrated into the host cell by recombinase-mediated cassette exchange (RMCE) between the donor sequence and the second plasmid via Cre-/cuc recombination. Further provided herein are host cells, vectors, and methods of producing a product related thereto.

Provided herein, in some aspects are high efficiency gene editing methods in bacterial cells using single-stranded annealing proteins and/or single-stranded binding proteins.

The present disclosure relates to serine proteases and variants thereof. Compositions containing the serine proteases are suitable for use in cleaning fabrics and hard surfaces, as well as in a variety of industrial applications.

The present disclosure relates to serine proteases and variants thereof. Compositions containing the serine proteases are suitable for use in cleaning fabrics and hard surfaces, as well as in a variety of industrial applications.

The present invention relates to a new isolated strain of Pseudomonas sp. deposited at the “Colección Española de Cultivos Tipo” (CECT) under the accession number CECT8708 or a mutant thereof; wherein said mutant is obtained using the CECT8708 of Pseudomonas sp. as starting material, maintains the dual bactericidal and fungicidal effects of CECT8708 and has a genome with an Average Nucleotide Identity (ANI) to the genome sequence SEQ ID NO: 1 of at least 99.8%. The strain of the invention has bactericidal and fungicidal effect.

The invention also relates to the inoculation product comprising the strain of the invention, the cell-free extract derived from the strain of the invention, and the composition comprising it and their uses in the control of bacteria and/or fungal infection in a plant. Also refers to a method to obtain the mutant strain of the strain of the invention.

The present invention relates to a new isolated strain of Pseudomonas sp. deposited at the “Colección Española de Cultivos Tipo” (CECT) under the accession number CECT8708 or a mutant thereof; wherein said mutant is obtained using the CECT8708 of Pseudomonas sp. as starting material, maintains the dual bactericidal and fungicidal effects of CECT8708 and has a genome with an Average Nucleotide Identity (ANI) to the genome sequence SEQ ID NO: 1 of at least 99.8%. The strain of the invention has bactericidal and fungicidal effect.

The invention also relates to the inoculation product comprising the strain of the invention, the cell-free extract derived from the strain of the invention, and the composition comprising it and their uses in the control of bacteria and/or fungal infection in a plant. Also refers to a method to obtain the mutant strain of the strain of the invention.