A formulation for a culture medium that is specifically designed to supplement a culture of differentiating hematopoietic stem cells (HSCs) with the factors necessary for high density manufacture of red blood cells (RBCs) while only requiring partial medium exchanges on a periodic basis. Further, the present disclosure identifies a factor, iron (III) citrate, also referred to as ferric citrate, that can supplant more commonly utilized sources of biological iron in culture medium for a more stable, more cost-effective medium formulation for superior vertical scalability.

Disclosed is a method for culturing mesenchymal stem cells, comprising culturing mesenchymal stem cells in a medium containing calcium in a concentration of from 2.1 to 3.8 mM and magnesium in a concentration of from 1.0 to 3.0 mM under a hypoxic condition of 2 to 5% oxygen. The culturing method can increase the population of mesenchymal stem cells even with a small number of passages by improving mesenchymal stem cells in proliferative capacity and viability. In addition, the mesenchymal stem cells prepared by the culturing method are effectively used to treat or improve a pulmonary disease.

A cardiomyocyte maturation medium comprises a base medium, one or more fatty acids such as palmitic acid, glucose and albumin. The one or more fatty acids and glucose are ideally present at a concentration ratio of about 1:10. Typically, the medium does not comprise TGF or insulin, or comprises minimal TGF and/or insulin. A cell culture vessel is provided that comprises opposed poles that facilitate force measurements of developing cardiac muscle.

Provided is a method for producing a liquid medium composition, including using a junction conduit structure 10 having a structure in which a first inlet conduit 11 and a second inlet conduit 12 are joined together to form an outlet conduit 13, and flowing a first liquid 1 containing a particular compound into the first inflow conduit 11 and flowing a second liquid 2 containing a linking substance into the second inlet conduit 12, and allowing the flows of the both liquids to join together to mix the both liquids, thus forming a liquid medium composition 3 containing structures formed by the particular compound linked via the linking substance dispersed therein while being flown through the outlet conduit 13.

The present invention relates to a chemically defined medium for eukaryotic cell culture, comprising water, at least one carbon source, one or more vitamins, one or more salts, one or more growth factors, one or more fatty acids, one or more buffer components, selenium and one or more further trace elements and its use in the culture of cancer stem cells, in particular tumorsphere culture of cancer stem cells.

Disclosed is a method for culturing mesenchymal stem cells, comprising culturing mesenchymal stem cells in a medium containing calcium in a concentration of from 2.1 to 3.8 mM and magnesium in a concentration of from 1.0 to 3.0 mM under a hypoxic condition of 2 to 5% oxygen. The culturing method can increase the population of mesenchymal stem cells even with a small number of passages by improving mesenchymal stem cells in proliferative capacity and viability. In addition, the mesenchymal stem cells prepared by the culturing method are effectively used not only as a safe cell therapeutic agent due to their lacking immunogenicity, but also as a cartilage regenerating medicine owing to their excellent secretion of cytokines.

The present disclosure encompasses methods for priming an allogeneic cultured keratinocyte composition for topical use.

Described herein are cell culture media, kits and methods for preparing cell culture media, and methods for culturing cells, for example, cells of the female reproductive tract, and tumor cells.

Disclosed herein are methods of culturing hematopoietic stem cells (HSCs) that enhance preservation of the HSCs self-renewal capacity and multipotency for extended periods of time. Specific embodiments comprise culturing methods that involve use of a unique low calcium media. Certain embodiments comprise methods that enable the maintenance of multipotent and self-renewing HSCs for over two weeks. Certain embodiments avoid the use of chemicals that have the risk of unexpected off-target effects or mutagenicity.

Disclosed are: a culture medium containing a specific growth factor and at least one phospholipid; a composition for preparation of the culture medium; a kit; and a method. A technique can be provided which uses a serum-free or low-serum culture medium and has a promoting effect on the proliferation of an animal cell comparable to the promoting effect obtained by the culture in a serum-containing culture medium.